UniProt functional annotation for P39605

UniProt code: P39605.

Organism:		Bacillus subtilis (strain 168).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
Function:		Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes. {ECO:0000269|PubMed:9836433}.
Catalytic activity:		Reaction=FMNH2 + NADP(+) = FMN + 2 H(+) + NADPH; Xref=Rhea:RHEA:21624, ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:57783, ChEBI:CHEBI:58210, ChEBI:CHEBI:58349; EC=1.5.1.38; Evidence={ECO:0000269|PubMed:9836433};
Biophysicochemical properties:		Kinetic parameters: KM=0.85 uM for NADPH (with nitrofurazone at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433}; KM=3.9 uM for NADPH (with FMN at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433}; KM=4.7 uM for FMN (with NADPH at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433}; KM=16.3 uM for nitrofurazone (with NADPH at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433}; Vmax=9 umol/min/mg enzyme with FMN as substrate (with NADPH at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433}; Vmax=97 umol/min/mg enzyme with nitrofurazone as substrate (with NADPH at pH 7 and at 23 degrees Celius) {ECO:0000269|PubMed:9836433};
Subunit:		Homodimer. {ECO:0000305|PubMed:9836433}.
Miscellaneous:		Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism. NADPH is more effective as an electron donor than NADH.
Similarity:		Belongs to the flavin oxidoreductase frp family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.