PDBsum entry 3msx

Protein binding

PDB id: 3msx

Contents

Protein chains

177 a.a.

192 a.a.

Ligands

GDP

MGF

Metals

_MG

Waters ×222

PDB id:

3msx

Name:

Protein binding

Title:

Crystal structure of rhoa.Gdp.Mgf3 in complex with gap domain of arhgap20

Structure:

Transforming protein rhoa. Chain: a. Synonym: rho cdna clone 12, h12. Engineered: yes. Mutation: yes. Rho gtpase-activating protein 20. Chain: b. Fragment: gap domain. Synonym: rho-type gtpase-activating protein 20.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: arh12, arha, rho12, rhoa. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: arhgap20, kiaa1391.

Resolution:

1.65Å R-factor: 0.196 R-free: 0.219

Authors:

D.Utepbergenov,D.R.Cooper,U.Derewenda,Z.S.Derewenda

Key ref:

D.Utepbergenov et al. Mechanism of molecular specificity of rhogap domains small gtpases of rhoa family.. To be published, .

Date:

29-Apr-10 Release date: 16-Mar-11

Protein chain

P61586  (RHOA_HUMAN) -  Transforming protein RhoA from Homo sapiens

Seq: 193 a.a.

Struc: 177 a.a.*

Protein chain

Q9P2F6  (RHG20_HUMAN) -  Rho GTPase-activating protein 20 from Homo sapiens

Seq: 1191 a.a.

Struc: 192 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + phosphate + H(+)

• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site