PDBsum entry 3mlp

Transcription/DNA

PDB id: 3mlp

Contents

Protein chains

337 a.a. *

303 a.a. *

DNA/RNA

Ligands

CIT ×5

Metals

_ZN ×4

Waters ×150

* Residue conservation analysis

PDB id:

3mlp

Name:

Transcription/DNA

Title:

Early b-cell factor 1 (ebf1) bound to DNA

Structure:

Transcription factor coe1. Chain: a, b, e, f. Fragment: DNA binding domain. Synonym: ebf1, o/e-1, oe-1, early b-cell factor. Engineered: yes. Mutation: yes. DNA (5'- d( Cp Tp Tp Tp Ap Tp Tp Cp Cp Cp Ap Tp Gp Gp Gp Ap Ap Tp Ap Ap Ap G)- 3').

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: ebf1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

2.80Å R-factor: 0.207 R-free: 0.264

Authors:

N.Treiber,T.Treiber,G.Zocher,R.Grosschedl

Key ref:

N.Treiber et al. (2010). Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins. Genes Dev, 24, 2270-2275. PubMed id: 20876732

Date:

17-Apr-10 Release date: 01-Dec-10

Protein chains

Q07802  (COE1_MOUSE) -  Transcription factor COE1 from Mus musculus

Seq: 591 a.a.

Struc: 337 a.a.*

Protein chains

Q07802  (COE1_MOUSE) -  Transcription factor COE1 from Mus musculus

Seq: 591 a.a.

Struc: 303 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-T-T-T-A-T-T-C-C-C-A-T-G-G-G-A-A-T-A-A-A-G 22 bases

C-T-T-T-A-T-T-C-C-C-A-T-G-G-G-A-A-T-A-A-A-G 22 bases

C-T-T-T-A-T-T-C-C-C-A-T-G-G-G-A-A-T-A-A-A-G 22 bases

C-T-T-T-A-T-T-C-C-C-A-T-G-G-G-A-A-T-A-A-A-G 22 bases

Genes Dev 24:2270-2275 (2010)

PubMed id: 20876732

Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins.

N.Treiber, T.Treiber, G.Zocher, R.Grosschedl.

ABSTRACT

No abstract given.