PDBsum entry 3mad

Lyase

PDB id: 3mad

Contents

Protein chains

451 a.a. *

Ligands

PO4 ×2

Waters ×350

* Residue conservation analysis

PDB id:

3mad

Name:

Lyase

Title:

Crystal structure of stspl (symmetric form)

Structure:

Sphingosine-1-phosphate lyase. Chain: a, b. Engineered: yes

Source:

Symbiobacterium thermophilum. Organism_taxid: 2734. Gene: sth1274. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å R-factor: 0.211 R-free: 0.247

Authors:

F.Bourquin,M.G.Grutter,G.Capitani

Key ref:

F.Bourquin et al. (2010). Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism. Structure, 18, 1054-1065. PubMed id: 20696404

Date:

23-Mar-10 Release date: 18-Aug-10

Protein chains

Q67PY4  (Q67PY4_SYMTH) -  Putative sphingosine-1-phosphate lyase from Symbiobacterium thermophilum (strain T / IAM 14863)

Seq: 507 a.a.

Struc: 451 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.2.27 - sphinganine-1-phosphate aldolase.
• Reaction: sphinganine 1-phosphate = hexadecanal + phosphoethanolamine

sphinganine 1-phosphate = hexadecanal + phosphoethanolamine (Bound ligand (Het Group name = PO4) matches with 62.50% similarity)

• Cofactor: Pyridoxal 5'-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Structure 18:1054-1065 (2010)

PubMed id: 20696404

Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.

F.Bourquin, H.Riezman, G.Capitani, M.G.Grütter.

ABSTRACT

No abstract given.