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UniProt functional annotation for P54860 | ||
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| UniProt code: P54860. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
E4 ubiquitin chain-elongation enzyme specifically involved in polyubiquitin chain assembly. Binds to CDC48 and elongates mono- and diubiquitinated ERAD substrates presented by the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex to a chain length of 4 to 6 ubiquitin moieties. Delivers these polyubiquitinated substrates to RAD23 and DSK2, which target them to the proteasome. Has E3 ubiquitin-protein ligase activity, accepting ubiquitin from its cognate E2 ubiquitin- conjugating enzyme UBC4. Enhances ubiquitination at 'Lys-48', but not at 'Lys-29' of the Ub moiety. Promotes ubiquitin chain elongation at 'Lys-48' on the DOA10 substrate PEX29. Also involved in the proteolytic processing of the ER-bound transcription factor SPT23. {ECO:0000269|PubMed:10089879, ECO:0000269|PubMed:15240124, ECO:0000269|PubMed:15652483, ECO:0000269|PubMed:18191224, ECO:0000269|PubMed:20159987}. |
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| Catalytic activity: | |
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:10089879}; |
| Pathway: | |
Protein modification; protein ubiquitination. {ECO:0000269|PubMed:10089879}. |
| Subunit: | |
Interacts with CDC48. Interacts with the ubiquitin-like domain of RAD23 and DSK2. Interacts with PEX29. {ECO:0000269|PubMed:10089879, ECO:0000269|PubMed:15121879, ECO:0000269|PubMed:16427015, ECO:0000269|PubMed:17890322, ECO:0000269|PubMed:20159987, ECO:0000269|PubMed:20427284}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. |
| Domain: | |
The U-box domain is required for the ubiquitin protein ligase activity. {ECO:0000269|PubMed:17890322}. |
| Miscellaneous: | |
Present with 2600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the ubiquitin conjugation factor E4 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.