PDBsum entry 3lq5

Transcription/inhibitor

PDB id: 3lq5

Contents

Protein chains

308 a.a. *

251 a.a. *

Ligands

SLQ

Waters ×9

* Residue conservation analysis

PDB id:

3lq5

Name:

Transcription/inhibitor

Title:

Structure of cdk9/cyclint in complex with s-cr8

Structure:

Cell division protein kinase 9. Chain: a. Fragment: kinase domain, unp residues 2-330. Synonym: cyclin-dependent kinase 9, serine/threonine-protein kinase pitalre, cell division cycle 2-like protein kinase 4, c-2k. Engineered: yes. Cyclin-t1. Chain: b. Fragment: cyclin domain, unp residues 2-259.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cdc2l4, cdk9. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: ccnt1.

Resolution:

3.00Å R-factor: 0.165 R-free: 0.215

Authors:

A.J.Hole,J.A.Endicott,S.Baumli

Key ref:

K.Bettayeb et al. (2010). CDK Inhibitors Roscovitine and CR8 Trigger Mcl-1 Down-Regulation and Apoptotic Cell Death in Neuroblastoma Cells. Genes Cancer, 1, 369-380. PubMed id: 21779453

Date:

08-Feb-10 Release date: 19-Jan-11

Protein chain

P50750  (CDK9_HUMAN) -  Cyclin-dependent kinase 9 from Homo sapiens

Seq: 372 a.a.

Struc: 308 a.a.*

Protein chain

O60563  (CCNT1_HUMAN) -  Cyclin-T1 from Homo sapiens

Seq: 726 a.a.

Struc: 251 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.7.11.22 - cyclin-dependent kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Enzyme class 3: Chain A: E.C.2.7.11.23 - [RNA-polymerase]-subunit kinase.
• Reaction: [DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Genes Cancer 1:369-380 (2010)

PubMed id: 21779453

CDK Inhibitors Roscovitine and CR8 Trigger Mcl-1 Down-Regulation and Apoptotic Cell Death in Neuroblastoma Cells.

K.Bettayeb, D.Baunbæk, C.Delehouze, N.Loaëc, A.J.Hole, S.Baumli, J.A.Endicott, S.Douc-Rasy, J.Bénard, N.Oumata, H.Galons, L.Meijer.

ABSTRACT

No abstract given.