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PDBsum entry 3lnh
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Cell adhesion
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PDB id
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3lnh
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References listed in PDB file
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Key reference
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Title
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Two-Step adhesive binding by classical cadherins.
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Authors
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O.J.Harrison,
F.Bahna,
P.S.Katsamba,
X.Jin,
J.Brasch,
J.Vendome,
G.Ahlsen,
K.J.Carroll,
S.R.Price,
B.Honig,
L.Shapiro.
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Ref.
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Nat Struct Biol, 2010,
17,
348-357.
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PubMed id
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Abstract
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Crystal structures of classical cadherins have revealed two dimeric
configurations. In the first, N-terminal beta-strands of EC1 domains 'swap'
between partner molecules. The second configuration (the 'X dimer'), also
observed for T-cadherin, is mediated by residues near the EC1-EC2 calcium
binding sites, and N-terminal beta-strands of partner EC1 domains, though held
adjacent, do not swap. Here we show that strand-swapping mutants of type I and
II classical cadherins form X dimers. Mutant cadherins impaired for X-dimer
formation show no binding in short-time frame surface plasmon resonance assays,
but in long-time frame experiments, they have homophilic binding affinities
close to that of wild type. Further experiments show that exchange between
monomers and dimers is slowed in these mutants. These results reconcile
apparently disparate results from prior structural studies and suggest that X
dimers are binding intermediates that facilitate the formation of strand-swapped
dimers.
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