PDBsum entry 3laf

Apoptosis

PDB id: 3laf

Contents

Protein chain

384 a.a. *

Ligands

NAG-NAG-BMA ×2

NAG ×2

SO4 ×12

Waters ×218

* Residue conservation analysis

PDB id:

3laf

Name:

Apoptosis

Title:

Structure of dcc, a netrin-1 receptor

Structure:

Deleted in colorectal cancer. Chain: a. Fragment: unp residues 39-421. Synonym: deleted in colorectal carcinoma, isoform cra_a. Engineered: yes

Source:

Rattus norvegicus. Rat. Organism_taxid: 10116. Gene: dcc, rcg_46581. Expressed in: trichoplusia ni. Expression_system_taxid: 7111.

Resolution:

2.40Å R-factor: 0.211 R-free: 0.231

Authors:

Q.Chen,J.-H.Liu,J.-H.Wang

Key ref:

Q.Chen et al. (2013). N-terminal horseshoe conformation of DCC is functionally required for axon guidance and might be shared by other neural receptors. J Cell Sci, 126, 186-195. PubMed id: 23038776 DOI: 10.1242/jcs.111278

Date:

06-Jan-10 Release date: 02-Mar-11

Protein chain

Q63155  (DCC_RAT) -  Netrin receptor DCC from Rattus norvegicus

Seq: 1445 a.a.

Struc: 384 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1242/jcs.111278

J Cell Sci 126:186-195 (2013)

PubMed id: 23038776

N-terminal horseshoe conformation of DCC is functionally required for axon guidance and might be shared by other neural receptors.

Q.Chen, X.Sun, X.H.Zhou, J.H.Liu, J.Wu, Y.Zhang, J.H.Wang.

ABSTRACT

Deleted in colorectal cancer (DCC) is a receptor for the axon guidance cues netrin-1 and draxin. The interactions between these guidance cues and DCC play a key role in the development of the nervous system. In the present study, we reveal the crystal structure of the N-terminal four Ig-like domains of DCC. The molecule folds into a horseshoe-like configuration. We demonstrate that this horseshoe conformation of DCC is required for guidance-cue-mediated axonal attraction. Structure-based mutations that disrupt the DCC horseshoe indeed impair its function. A comparison of the DCC horseshoe with previously described horseshoe structures has revealed striking conserved structural features and important sequence signatures. Using these signatures, a genome-wide search allows us to predict the N-terminal horseshoe arrangement in a number of other cell surface receptors, nearly all of which function in the nervous system. The N-terminal horseshoe appears to be evolutionally selected as a platform for neural receptors.