PDBsum entry 3k9p

Ligase/signaling protein

PDB id: 3k9p

Contents

Protein chains

199 a.a. *

72 a.a. *

* Residue conservation analysis

PDB id:

3k9p

Name:

Ligase/signaling protein

Title:

The crystal structure of e2-25k and ubiquitin complex

Structure:

Ubiquitin-conjugating enzyme e2 k. Chain: a. Synonym: ubiquitin-conjugating enzyme e2-25 kda, e2(25k), e2-25k, ubiquitin-protein ligase, ubiquitin carrier protein, huntingtin- interacting protein 2, hip-2. Engineered: yes. Ubiquitin. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2k, hip2, lig. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.80Å R-factor: 0.232 R-free: 0.296

Authors:

G.B.Kang,S.Ko,S.M.Song,W.Lee,S.H.Eom

Key ref:

S.Ko et al. (2010). Structural basis of E2-25K/UBB+1 interaction leading to proteasome inhibition and neurotoxicity. J Biol Chem, 285, 36070-36080. PubMed id: 20826778

Date:

16-Oct-09 Release date: 08-Sep-10

Protein chain

P61086  (UBE2K_HUMAN) -  Ubiquitin-conjugating enzyme E2 K from Homo sapiens

Seq: 200 a.a.

Struc: 199 a.a.

Protein chain

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 72 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

J Biol Chem 285:36070-36080 (2010)

PubMed id: 20826778

Structural basis of E2-25K/UBB+1 interaction leading to proteasome inhibition and neurotoxicity.

S.Ko, G.B.Kang, S.M.Song, J.G.Lee, D.Y.Shin, J.H.Yun, Y.Sheng, C.Cheong, Y.H.Jeon, Y.K.Jung, C.H.Arrowsmith, G.V.Avvakumov, S.Dhe-Paganon, Y.J.Yoo, S.H.Eom, W.Lee.

ABSTRACT

No abstract given.