spacer
spacer

PDBsum entry 3k2f

Go to PDB code: 
Top Page protein ligands metals links
Lyase PDB id
3k2f
Jmol
Contents
Protein chain
256 a.a.
Ligands
NKX
Metals
_ZN
_HG
Waters ×120

References listed in PDB file
Key reference
Title Nitric oxide-Donating carbonic anhydrase inhibitors for the treatment of open-Angle glaucoma
Authors R.M.Steele, F.Benedini, S.Biondi, V.Borghi, L.Carzaniga, F.Impagnatiello, D.Miglietta, W.K.Chong, R.Rajapakse, A.Cecchi, C.Temperini, C.T.Supuran.
Ref. Bioorg.Med.Chem.Lett., 2009, 19, 6565-6570.
PubMed id 19854054
Abstract
Novel bi-functional compounds with a nitric oxide (NO)-releasing moiety bound to a dorzolamide scaffold were investigated. Several compounds were synthesized and their activity as selective carbonic anhydrase inhibitors (CAI) evaluated in vitro on recombinant hCA type I, II and IV enzyme isoforms where they showed different degrees of potency and selectivity to hCA II. A high resolution X-ray crystal structure for the CA II adduct with 8 confirmed the high affinity of this class of compounds for the enzyme. Compounds 4, 6, and 8 showed highly potent and efficacious NO-mediated properties as assessed by their vascular relaxant effect on methoxamine-precontracted rabbit aortic rings. Finally, compounds 4 and 6 exerted potent intraocular pressure (IOP) lowering effects in vivo in normotensive rabbits thereby anticipating their potential for the treatment of hypertensive glaucoma.
PROCHECK
Go to PROCHECK summary
 Headers

 

spacer

spacer