PDBsum entry 3jy9

Transferase

PDB id: 3jy9

Contents

Protein chain

284 a.a. *

Ligands

JZH

Metals

_NA

Waters ×176

* Residue conservation analysis

PDB id:

3jy9

Name:

Transferase

Title:

Janus kinase 2 inhibitors

Structure:

Tyrosine-protein kinase jak2. Chain: a. Fragment: unp residue 842-1130, protein kinase 2 domain. Synonym: janus kinase 2, jak-2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: jak2. Expressed in: baculovirus. Expression_system_taxid: 10469.

Resolution:

2.10Å R-factor: 0.201 R-free: 0.271

Authors:

H.J.Zuccola,M.W.Ledeboer,A.C.Pierce

Key ref:

T.Wang et al. (2009). Janus kinase 2 inhibitors. Synthesis and characterization of a novel polycyclic azaindole. J Med Chem, 52, 7938-7941. PubMed id: 20014869

Date:

21-Sep-09 Release date: 01-Dec-09

Protein chain

O60674  (JAK2_HUMAN) -  Tyrosine-protein kinase JAK2 from Homo sapiens

Seq: 1132 a.a.

Struc: 284 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Med Chem 52:7938-7941 (2009)

PubMed id: 20014869

Janus kinase 2 inhibitors. Synthesis and characterization of a novel polycyclic azaindole.

T.Wang, J.P.Duffy, J.Wang, S.Halas, F.G.Salituro, A.C.Pierce, H.J.Zuccola, J.R.Black, J.K.Hogan, S.Jepson, D.Shlyakter, S.Mahajan, Y.Gu, T.Hoock, M.Wood, B.F.Furey, J.D.Frantz, L.M.Dauffenbach, U.A.Germann, B.Fan, M.Namchuk, Y.L.Bennani, M.W.Ledeboer.

ABSTRACT

The synthesis and characterization of a novel polycyclic azaindole based derivative is disclosed, and its binding to JAK2 is described. The compound is further evaluated for its ability to block the EPO/JAK2 signaling cascade in vitro and in vivo.