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PDBsum entry 3jsv
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Signaling protein/transcription
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PDB id
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3jsv
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the nemo ubiquitin-Binding domain in complex with lys 63-Linked di-Ubiquitin.
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Authors
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A.Yoshikawa,
Y.Sato,
M.Yamashita,
H.Mimura,
A.Yamagata,
S.Fukai.
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Ref.
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Febs Lett, 2009,
583,
3317-3322.
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PubMed id
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Abstract
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NEMO is essential for activation of the NF-kappaB signaling pathway, which is
regulated by ubiquitination of proteins. The C-terminal leucine zipper of NEMO
and its adjacent coiled-coil region (CC2-LZ) reportedly bind to linear ubiquitin
chains with 1 microM affinity and to Lys 63-linked chains with 100 microM
affinity. Here we report the crystal structure of the CC2-LZ region of mouse
NEMO in complex with Lys 63-linked di-ubiquitin (K63-Ub(2)) at 2.7A resolution.
The ubiquitin-binding region consists of a 130A-long helix and forms a parallel
coiled-coil dimer. The Ile 44-centered hydrophobic patch of ubiquitin is
recognized in the middle of the NEMO ubiquitin-binding region. NEMO interacts
with each K63-Ub(2)via a single ubiquitin-binding site, consistent with low
affinity binding with K63-Ub(2).
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