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PDBsum entry 3jb9
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RNA binding protein/RNA
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PDB id
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3jb9
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Contents |
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1964 a.a.
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904 a.a.
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96 a.a.
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98 a.a.
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82 a.a.
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95 a.a.
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76 a.a.
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73 a.a.
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73 a.a.
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373 a.a.
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293 a.a.
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207 a.a.
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132 a.a.
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430 a.a.
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426 a.a.
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261 a.a.
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255 a.a.
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300 a.a.
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155 a.a.
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144 a.a.
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148 a.a.
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90 a.a.
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161 a.a.
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575 a.a.
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573 a.a.
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1195 a.a.
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160 a.a.
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89 a.a.
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80 a.a.
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74 a.a.
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87 a.a.
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References listed in PDB file
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Key reference
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Title
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Structure of a yeast spliceosome at 3.6-Angstrom resolution.
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Authors
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C.Yan,
J.Hang,
R.Wan,
M.Huang,
C.C.Wong,
Y.Shi.
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Ref.
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Science, 2015,
349,
1182-1191.
[DOI no: ]
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PubMed id
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Abstract
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Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the
spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs),
NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated
enzymes and cofactors. Here, we report the three-dimensional structure of a
Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by
means of single-particle cryogenic electron microscopy. This spliceosome
contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron
lariat. The atomic model includes 10,574 amino acids from 37 proteins and four
RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons.
Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5
snRNP, forms a central scaffold and anchors the catalytic center. Both the
morphology and the placement of protein components appear to have evolved to
facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution
structure of a central spliceosome provides a molecular framework for
mechanistic understanding of pre-mRNA splicing.
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