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PDBsum entry 3j9i
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Contents |
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(+ 8 more)
224 a.a.
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(+ 8 more)
203 a.a.
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References listed in PDB file
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Key reference
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Title
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Electron counting and beam-Induced motion correction enable near-Atomic-Resolution single-Particle cryo-Em.
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Authors
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X.Li,
P.Mooney,
S.Zheng,
C.R.Booth,
M.B.Braunfeld,
S.Gubbens,
D.A.Agard,
Y.Cheng.
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Ref.
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Nat Methods, 2013,
10,
584-590.
[DOI no: ]
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PubMed id
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Abstract
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In recent work with large high-symmetry viruses, single-particle electron
cryomicroscopy (cryo-EM) has achieved the determination of
near-atomic-resolution structures by allowing direct fitting of atomic models
into experimental density maps. However, achieving this goal with smaller
particles of lower symmetry remains challenging. Using a newly developed single
electron-counting detector, we confirmed that electron beam-induced motion
substantially degrades resolution, and we showed that the combination of rapid
readout and nearly noiseless electron counting allow image blurring to be
corrected to subpixel accuracy, restoring intrinsic image information to high
resolution (Thon rings visible to ∼3 Å). Using this approach, we determined a
3.3-Å-resolution structure of an ∼700-kDa protein with D7 symmetry, the
Thermoplasma acidophilum 20S proteasome, showing clear side-chain density. Our
method greatly enhances image quality and data acquisition efficiency-key
bottlenecks in applying near-atomic-resolution cryo-EM to a broad range of
protein samples.
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Secondary reference #1
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Title
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Side-Chain-Directed model and map validation for 3d e cryomicroscopy
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Authors
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B.A.Barad,
N.Echols,
R.Y.-R.Wang,
Y.Cheng,
F.Dimaio,
P.D.Aj.S.Fraser.
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Ref.
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TO BE PUBLISHED ...
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