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PDBsum entry 3j9e
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Viral protein
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PDB id
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3j9e
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References listed in PDB file
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Key reference
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Title
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Atomic model of a nonenveloped virus reveals ph sensors for a coordinated process of cell entry.
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Authors
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X.Zhang,
A.Patel,
C.C.Celma,
X.Yu,
P.Roy,
Z.H.Zhou.
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Ref.
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Nat Struct Biol, 2016,
23,
74-80.
[DOI no: ]
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PubMed id
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Abstract
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Viruses sense environmental cues such as pH to engage in membrane interactions
for cell entry during infection, but how nonenveloped viruses sense pH is
largely undefined. Here, we report both high- and low-pH structures of
bluetongue virus (BTV), which enters cells via a two-stage endosomal process.
The receptor-binding protein VP2 possesses a zinc finger that may function to
maintain VP2 in a metastable state and a conserved His866, which senses
early-endosomal pH. The membrane-penetration protein VP5 has three domains:
dagger, unfurling and anchoring. Notably, the β-meander motif of the anchoring
domain contains a histidine cluster that can sense late-endosomal pH and also
possesses four putative membrane-interaction elements. Exposing BTV to low pH
detaches VP2 and dramatically refolds the dagger and unfurling domains of VP5.
Our biochemical and structure-guided-mutagenesis studies support these
coordinated pH-sensing mechanisms.
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