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PDBsum entry 3j5m
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Viral protein/immune system
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PDB id
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3j5m
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Contents |
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415 a.a.
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63 a.a.
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208 a.a.
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225 a.a.
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PDB id:
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| Name: |
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Viral protein/immune system
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Title:
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Cryo-em structure of the bg505 sosip.664 HIV-1 env trimer with 3 pgv04 fabs
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Structure:
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Bg505 sosip gp120. Chain: a, e, i. Fragment: unp residues 30-504. Engineered: yes. Mutation: yes. Bg505 sosip gp41. Chain: b, f, j. Fragment: see remark 999. Engineered: yes.
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Source:
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Human immunodeficiency virus 1. Organism_taxid: 11676. Gene: env. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek. Homo sapiens. Human. Organism_taxid: 9606.
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Authors:
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D.Lyumkis,J.-P.Julien,I.A.Wilson,A.B.Ward
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Key ref:
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D.Lyumkis
et al.
(2013).
Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.
Science,
342,
1484-1490.
PubMed id:
DOI:
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Date:
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26-Oct-13
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Release date:
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13-Nov-13
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PROCHECK
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Headers
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References
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Q2N0S6
(Q2N0S6_HV1) -
Envelope glycoprotein gp160 from Human immunodeficiency virus type 1
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Seq:
Struc:
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860 a.a.
415 a.a.*
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No UniProt id for this chain
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DOI no:
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Science
342:1484-1490
(2013)
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PubMed id:
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Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.
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D.Lyumkis,
J.P.Julien,
N.de Val,
A.Cupo,
C.S.Potter,
P.J.Klasse,
D.R.Burton,
R.W.Sanders,
J.P.Moore,
B.Carragher,
I.A.Wilson,
A.B.Ward.
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ABSTRACT
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The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites
and membrane fusion machinery that introduce the viral genome into the host
cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a
focus for rational vaccine design. We present a cryo-electron microscopy
reconstruction and structural model of a cleaved, soluble Env trimer (termed
BG505 SOSIP.664 gp140) in complex with a CD4 binding site (CD4bs) bnAb, PGV04,
at 5.8 angstrom resolution. The structure reveals the spatial arrangement of Env
components, including the V1/V2, V3, HR1, and HR2 domains, as well as shielding
glycans. The structure also provides insights into trimer assembly, gp120-gp41
interactions, and the CD4bs epitope cluster for bnAbs, which covers a more
extensive area and defines a more complex site of vulnerability than previously
described.
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Links
