PDBsum entry 3j5l

Ribosome/antibiotic

PDB id: 3j5l

Contents

Protein chains

56 a.a.

50 a.a.

46 a.a.

64 a.a.

38 a.a.

8 a.a.*

271 a.a.

209 a.a.

201 a.a.

177 a.a.

176 a.a.

56 a.a.

141 a.a.

142 a.a.

122 a.a.

143 a.a.

136 a.a.

120 a.a.

116 a.a.

114 a.a.

117 a.a.

103 a.a.

110 a.a.

93 a.a.

102 a.a.

94 a.a.

79 a.a.

77 a.a.

63 a.a.

58 a.a.

DNA/RNA

Ligands

_DC-MA6

UNL

ERY

* C-alpha coords only

PDB id:

3j5l

Name:

Ribosome/antibiotic

Title:

Structure of the e. Coli 50s subunit with ermbl nascent chain

Structure:

50s ribosomal protein l32. Chain: 0. 50s ribosomal protein l33. Chain: 1. 50s ribosomal protein l34. Chain: 2. 50s ribosomal protein l35. Chain: 3. 50s ribosomal protein l36.

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k-12. Synthetic: yes. Streptococcus sanguinis. Organism_taxid: 1305. Strain: k-12

Authors:

S.Arenz,H.Ramu,P.Gupta,O.Berninghausen,R.Beckmann,N.Vazquez-Laslop, A.S.Mankin,D.N.Wilson

Key ref:

S.Arenz et al. (2014). Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide. Nat Commun, 5, 3501. PubMed id: 24662426 DOI: 10.1038/ncomms4501

Date:

23-Oct-13 Release date: 26-Mar-14

Protein chain

P0A7N4  (RL32_ECOLI) -  Large ribosomal subunit protein bL32 from Escherichia coli (strain K12)

Seq: 57 a.a.

Struc: 56 a.a.

Protein chain

P0A7N9  (RL33_ECOLI) -  Large ribosomal subunit protein bL33 from Escherichia coli (strain K12)

Seq: 55 a.a.

Struc: 50 a.a.

Protein chain

P0A7P5  (RL34_ECOLI) -  Large ribosomal subunit protein bL34 from Escherichia coli (strain K12)

Seq: 46 a.a.

Struc: 46 a.a.

Protein chain

P0A7Q1  (RL35_ECOLI) -  Large ribosomal subunit protein bL35 from Escherichia coli (strain K12)

Seq: 65 a.a.

Struc: 64 a.a.

Protein chain

P0A7Q6  (RL36_ECOLI) -  Large ribosomal subunit protein bL36A from Escherichia coli (strain K12)

Seq: 38 a.a.

Struc: 38 a.a.

Protein chain

No UniProt id for this chain

Struc: 8 a.a.

Protein chain

P60422  (RL2_ECOLI) -  Large ribosomal subunit protein uL2 from Escherichia coli (strain K12)

Seq: 273 a.a.

Struc: 271 a.a.

Protein chain

P60438  (RL3_ECOLI) -  Large ribosomal subunit protein uL3 from Escherichia coli (strain K12)

Seq: 209 a.a.

Struc: 209 a.a.

Protein chain

P60723  (RL4_ECOLI) -  Large ribosomal subunit protein uL4 from Escherichia coli (strain K12)

Seq: 201 a.a.

Struc: 201 a.a.

Protein chain

P62399  (RL5_ECOLI) -  Large ribosomal subunit protein uL5 from Escherichia coli (strain K12)

Seq: 179 a.a.

Struc: 177 a.a.

Protein chain

P0AG55  (RL6_ECOLI) -  Large ribosomal subunit protein uL6 from Escherichia coli (strain K12)

Seq: 177 a.a.

Struc: 176 a.a.

Protein chain

P0A7R1  (RL9_ECOLI) -  Large ribosomal subunit protein bL9 from Escherichia coli (strain K12)

Seq: 149 a.a.

Struc: 56 a.a.

Protein chain

P0A7J7  (RL11_ECOLI) -  Large ribosomal subunit protein uL11 from Escherichia coli (strain K12)

Seq: 142 a.a.

Struc: 141 a.a.

Protein chain

P0AA10  (RL13_ECOLI) -  Large ribosomal subunit protein uL13 from Escherichia coli (strain K12)

Seq: 142 a.a.

Struc: 142 a.a.

Protein chain

P0ADY3  (RL14_ECOLI) -  Large ribosomal subunit protein uL14 from Escherichia coli (strain K12)

Seq: 123 a.a.

Struc: 122 a.a.

Protein chain

P02413  (RL15_ECOLI) -  Large ribosomal subunit protein uL15 from Escherichia coli (strain K12)

Seq: 144 a.a.

Struc: 143 a.a.

Protein chain

P0ADY7  (RL16_ECOLI) -  Large ribosomal subunit protein uL16 from Escherichia coli (strain K12)

Seq: 136 a.a.

Struc: 136 a.a.

Protein chain

P0AG44  (RL17_ECOLI) -  Large ribosomal subunit protein bL17 from Escherichia coli (strain K12)

Seq: 127 a.a.

Struc: 120 a.a.

Protein chain

P0C018  (RL18_ECOLI) -  Large ribosomal subunit protein uL18 from Escherichia coli (strain K12)

Seq: 117 a.a.

Struc: 116 a.a.

Protein chain

P0A7K6  (RL19_ECOLI) -  Large ribosomal subunit protein bL19 from Escherichia coli (strain K12)

Seq: 115 a.a.

Struc: 114 a.a.

Protein chain

P0A7L3  (RL20_ECOLI) -  Large ribosomal subunit protein bL20 from Escherichia coli (strain K12)

Seq: 118 a.a.

Struc: 117 a.a.

Protein chain

P0AG48  (RL21_ECOLI) -  Large ribosomal subunit protein bL21 from Escherichia coli (strain K12)

Seq: 103 a.a.

Struc: 103 a.a.

Protein chain

P61175  (RL22_ECOLI) -  Large ribosomal subunit protein uL22 from Escherichia coli (strain K12)

Seq: 110 a.a.

Struc: 110 a.a.

Protein chain

P0ADZ0  (RL23_ECOLI) -  Large ribosomal subunit protein uL23 from Escherichia coli (strain K12)

Seq: 100 a.a.

Struc: 93 a.a.

Protein chain

P60624  (RL24_ECOLI) -  Large ribosomal subunit protein uL24 from Escherichia coli (strain K12)

Seq: 104 a.a.

Struc: 102 a.a.

Protein chain

P68919  (RL25_ECOLI) -  Large ribosomal subunit protein bL25 from Escherichia coli (strain K12)

Seq: 94 a.a.

Struc: 94 a.a.

Protein chain

P0A7L8  (RL27_ECOLI) -  Large ribosomal subunit protein bL27 from Escherichia coli (strain K12)

Seq: 85 a.a.

Struc: 79 a.a.

Protein chain

P0A7M2  (RL28_ECOLI) -  Large ribosomal subunit protein bL28 from Escherichia coli (strain K12)

Seq: 78 a.a.

Struc: 77 a.a.

Protein chain

P0A7M6  (RL29_ECOLI) -  Large ribosomal subunit protein uL29 from Escherichia coli (strain K12)

Seq: 63 a.a.

Struc: 63 a.a.

Protein chain

P0AG51  (RL30_ECOLI) -  Large ribosomal subunit protein uL30 from Escherichia coli (strain K12)

Seq: 59 a.a.

Struc: 58 a.a.

DNA/RNA chains

C-C-A 3 bases

G-G-U-U-A-A-C-G-A-C-U-A-A-G-C-G-U-A-C-A-C-G-G-U-G-G-A-U-G-C-C-C-U-G-G-C-A-G-U- ... 2853 bases

G-C-C-U-G-C-G-G-C-C-G-U-A-G-C-G-C-G-G-U-G-G-U-C-C-C-A-C-C-U-G-A-C-C-C-C-A-U-G- 117 bases

Key reference

DOI no: 10.1038/ncomms4501

Nat Commun 5:3501 (2014)

PubMed id: 24662426

Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide.

S.Arenz, H.Ramu, P.Gupta, O.Berninghausen, R.Beckmann, N.Vázquez-Laslop, A.S.Mankin, D.N.Wilson.

ABSTRACT

No abstract given.