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PDBsum entry 3j2y
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Immune system
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PDB id
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3j2y
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References listed in PDB file
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Key reference
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Title
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Structural analyses at pseudo atomic resolution of chikungunya virus and antibodies show mechanisms of neutralization.
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Authors
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S.Sun,
Y.Xiang,
W.Akahata,
H.Holdaway,
P.Pal,
X.Zhang,
M.S.Diamond,
G.J.Nabel,
M.G.Rossmann.
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Ref.
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Elife, 2013,
2,
e00435.
[DOI no: ]
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PubMed id
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Abstract
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A 5.3 Å resolution, cryo-electron microscopy (cryoEM) map of Chikungunya
virus-like particles (VLPs) has been interpreted using the previously published
crystal structure of the Chikungunya E1-E2 glycoprotein heterodimer. The
heterodimer structure was divided into domains to obtain a good fit to the
cryoEM density. Differences in the T = 4 quasi-equivalent heterodimer components
show their adaptation to different environments. The spikes on the icosahedral
3-fold axes and those in general positions are significantly different, possibly
representing different phases during initial generation of fusogenic E1 trimers.
CryoEM maps of neutralizing Fab fragments complexed with VLPs have been
interpreted using the crystal structures of the Fab fragments and the VLP
structure. Based on these analyses the CHK-152 antibody was shown to stabilize
the viral surface, hindering the exposure of the fusion-loop, likely
neutralizing infection by blocking fusion. The CHK-9, m10 and m242 antibodies
surround the receptor-attachment site, probably inhibiting infection by blocking
cell attachment. DOI:http://dx.doi.org/10.7554/eLife.00435.001.
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