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PDBsum entry 3j1n
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Contents |
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1412 a.a.
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1094 a.a.
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266 a.a.
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177 a.a.
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214 a.a.
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84 a.a.
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171 a.a.
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133 a.a.
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118 a.a.
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65 a.a.
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114 a.a.
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46 a.a.
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References listed in PDB file
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Key reference
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Title
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Interaction of the mediator head module with RNA polymerase ii.
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Authors
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G.Cai,
Y.L.Chaban,
T.Imasaki,
J.A.Kovacs,
G.Calero,
P.A.Penczek,
Y.Takagi,
F.J.Asturias.
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Ref.
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Structure, 2012,
20,
899-910.
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PubMed id
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Abstract
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Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout
eukaryotes, plays an essential role in control of gene expression by conveying
regulatory signals that influence the activity of the preinitiation complex.
However, the precise mode of interaction between Mediator and RNA polymerase II
(RNAPII), and the mechanism of regulation by Mediator remain elusive. We used
cryo-electron microscopy and reconstituted in vitro transcription assays to
characterize a transcriptionally-active complex including the Mediator Head
module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB,
TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII,
affecting its conformation and function.
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