UniProt functional annotation for P0ADY3

UniProt codes: P0ADY3, P02411.

Organism: Escherichia coli (strain K12).
Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
 
Function: This protein binds directly to 23S ribosomal RNA. In the E.coli 70S ribosome (PubMed:12809609) it has been modeled to make two contacts with the 16S rRNA of the 30S subunit, forming part of bridges B5 and B8, connecting the 2 subunits. Although the protein undergoes significant rotation during the transition from an initiation to and EF-G bound state, the bridges remain stable. In the 3.5 A resolved structures (PubMed:16272117) L14 and L19 interact and together make contact with the 16S rRNA in bridges B5 and B8. {ECO:0000269|PubMed:12809609, ECO:0000269|PubMed:16272117, ECO:0000269|PubMed:22829778}.
 
Function: Can also interact with RsfS, in this case bridge B8 probably cannot form, and the 30S and 50S ribosomal subunits do not associate, which represses translation. {ECO:0000269|PubMed:22829778}.
 
Subunit: Part of the 50S ribosomal subunit. Contacts L19 (PubMed:2665813 and PubMed:16272117). Forms two bridges to the 30S subunit in the 70S ribosome, contacting the 16S rRNA. Can also contact RsfS, which then probably inhibits ribosomal subunit association.
Mass spectrometry: Mass=13540.2; Method=MALDI; Range=1-123; Evidence={ECO:0000269|PubMed:10094780};
Similarity: Belongs to the ribosomal protein L14P family. {ECO:0000255|HAMAP-Rule:MF_01367}.

Annotations taken from UniProtKB at the EBI.