UniProt functional annotation for P62399

UniProt code: P62399.

Organism: Escherichia coli (strain K12).
Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
 
Function: This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. Its 5S rRNA binding is significantly enhanced in the presence of L18.
 
Function: In the 70S ribosome in the initiation state (PubMed:12809609) was modeled to contact protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; the protein- protein contacts between S13 and L5 in B1b change in the model with bound EF-G implicating this bridge in subunit movement (PubMed:12809609 and PubMed:18723842). In the two 3.5 A resolved ribosome structures (PubMed:16272117) the contacts between L5, S13 and S19 are different, confirming the dynamic nature of this interaction. {ECO:0000269|PubMed:12809609, ECO:0000269|PubMed:16272117}.
 
Function: Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome- bound tRNAs.
 
Subunit: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA; cross-links to the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome, contacting protein S13 and S19.
Mass spectrometry: Mass=20169.8; Method=MALDI; Range=2-179; Evidence={ECO:0000269|PubMed:10094780};
Similarity: Belongs to the ribosomal protein L5P family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.