UniProt functional annotation for P0C018

UniProt code: P0C018.

Organism: Escherichia coli (strain K12).
Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
 
Function: This is one of the proteins that mediates the attachment of the 5S rRNA subcomplex onto the large ribosomal subunit where it forms part of the central protuberance. Binds stably to 5S rRNA; increases binding abilities of L5 in a cooperative fashion; both proteins together confer 23S rRNA binding. The 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. {ECO:0000269|PubMed:353728}.
 
Subunit: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.
Domain: The basic N-terminus is not necessary for binding to 5S rRNA. It is however required for cooperative binding of L5 and L18 to 5S rRNA as well as for binding of the 5S rRNA/L5/L18 complex to the 23S rRNA.
Ptm: The protein has been shown to contain a phosphoserine, which was required for the protein to bind to 5S rRNA (PubMed:10529214). However, the presence of this phosphoserine is controversial, and it has not been seen by mass spectrometry. {ECO:0000269|PubMed:10529214}.
Mass spectrometry: Mass=12769.8; Method=MALDI; Range=1-117; Evidence={ECO:0000269|PubMed:10094780};
Similarity: Belongs to the ribosomal protein L18P family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.