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PDBsum entry 3iuy

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
3iuy
Jmol
Contents
Protein chain
213 a.a. *
Ligands
AMP ×2
Metals
_CL ×2
Waters ×155
* Residue conservation analysis
PDB id:
3iuy
Name: Hydrolase
Title: Crystal structure of ddx53 dead-box domain
Structure: Probable atp-dependent RNA helicase ddx53. Chain: a, b. Fragment: unp residues 204-430, helicase atp-binding domain synonym: dead box protein 53, dead box protein cage, cancer associated gene protein, cancer/testis antigen 26, ct26. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ddx53, cage. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.40Å     R-factor:   0.204     R-free:   0.251
Authors: P.Schutz,T.Karlberg,R.Collins,C.H.Arrowsmith,H.Berglund,C.Bo Edwards,S.Flodin,A.Flores,S.Graslund,M.Hammarstrom,A.Johans I.Johansson,A.Kallas,P.Kraulis,T.Kotenyova,A.Kotzsch,N.Mark M.Moche,T.K.Nielsen,P.Nordlund,T.Nyman,C.Persson,A.K.Roos, M.I.Siponen,L.Svensson,A.G.Thorsell,L.Tresaugues,S.Van Den E.Wahlberg,J.Weigelt,M.Welin,M.Wisniewska,H.M.Schuler,Struc Genomics Consortium (Sgc)
Key ref: P.Schütz et al. (2010). Comparative structural analysis of human DEAD-box RNA helicases. PLoS One, 5, 12791-12791. PubMed id: 20941364
Date:
31-Aug-09     Release date:   20-Oct-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q86TM3  (DDX53_HUMAN) -  Probable ATP-dependent RNA helicase DDX53
Seq:
Struc:
 
Seq:
Struc:
631 a.a.
213 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.6.4.13  - Rna helicase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate
ATP
+ H(2)O
=
ADP
Bound ligand (Het Group name = AMP)
matches with 85.00% similarity
+ phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     nucleic acid binding     3 terms  

 

 
    reference    
 
 
PLoS One 5:12791-12791 (2010)
PubMed id: 20941364  
 
 
Comparative structural analysis of human DEAD-box RNA helicases.
P.Schütz, T.Karlberg, S.van den Berg, R.Collins, L.Lehtiö, M.Högbom, L.Holmberg-Schiavone, W.Tempel, H.W.Park, M.Hammarström, M.Moche, A.G.Thorsell, H.Schüler.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22940866 A.L.Mallam, M.Del Campo, B.Gilman, D.J.Sidote, and A.M.Lambowitz (2012).
Structural basis for RNA-duplex recognition and unwinding by the DEAD-box helicase Mss116p.
  Nature, 490, 121-125.
PDB codes: 4db2 4db4
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.