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PDBsum entry 3ije
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protein ligands metals Protein-protein interface(s) links
Protein binding PDB id
3ije

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
938 a.a. *
695 a.a. *
Ligands
NAG-NAG-BMA-MAN-
MAN
NAG-NAG ×6
NAG-NAG-BMA-MAN-
MAN-MAN
NAG-NAG-BMA ×2
NAG ×5
Metals
_CA ×6
* Residue conservation analysis
PDB id:
3ije
Name: Protein binding
Title: Crystal structure of the complete integrin alhavbeta3 ectodomain plus an alpha/beta transmembrane fragment
Structure: Integrin alpha-v. Chain: a. Synonym: vitronectin receptor subunit alpha, integrin alpha-v heavy chain, integrin alpha-v light chain. Engineered: yes. Integrin beta-3. Chain: b. Synonym: platelet membrane glycoprotein iiia, gpiiia. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: alphav. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: itgb3, gp3a.
Resolution:
2.90Å     R-factor:   0.246     R-free:   0.285
Authors: J.-P.Xiong,B.Mahalingham,X.Rui,B.T.Hyman,S.L.Goodman,M.A.Arnaout
Key ref: J.P.Xiong et al. (2009). Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment. J Cell Biol, 186, 589-600. PubMed id: 19704023
Date:
04-Aug-09     Release date:   29-Sep-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06756  (ITAV_HUMAN) -  Integrin alpha-V from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1048 a.a.
938 a.a.
Protein chain
Pfam   ArchSchema ?
P05106  (ITB3_HUMAN) -  Integrin beta-3 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		788 a.a.
695 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
J Cell Biol 186:589-600 (2009)
PubMed id: 19704023  
 
 
Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.
J.P.Xiong, B.Mahalingham, J.L.Alonso, L.A.Borrelli, X.Rui, S.Anand, B.T.Hyman, T.Rysiok, D.Müller-Pompalla, S.L.Goodman, M.A.Arnaout.
 
  ABSTRACT  
 
We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and defines the alpha-beta interface between IE2 (EGF-like 2) and the thigh domains. Modifying this interface by site-directed mutagenesis leads to robust integrin activation. Fluorescent lifetime imaging microscopy of inactive full-length alphaVbeta3 on live cells yields a donor-membrane acceptor distance, which is consistent with the bent conformation and does not change in the activated integrin. These data are the first direct demonstration of conformational coupling of the integrin leg and head domains, identify the IE2-thigh interface as a critical steric barrier in integrin activation, and suggest that inside-out activation in intact cells may involve conformational changes other than the postulated switch to a genu-linear state.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20831445 I.Azimi, J.W.Wong, and P.J.Hogg (2011).
Control of mature protein function by allosteric disulfide bonds.
  Antioxid Redox Signal, 14, 113-126.  
21216149 N.J.Anthis, and I.D.Campbell (2011).
The tail of integrin activation.
  Trends Biochem Sci, 36, 191-198.  
21087420 P.Pinon, and B.Wehrle-Haller (2011).
Integrins: versatile receptors controlling melanocyte adhesion, migration and proliferation.
  Pigment Cell Melanoma Res, 24, 282-294.  
21379327 W.Chen, J.Lou, J.Hsin, K.Schulten, S.C.Harvey, and C.Zhu (2011).
Molecular dynamics simulations of forced unbending of integrin α(v)β₃.
  PLoS Comput Biol, 7, e1001086.  
19897481 A.Nogales, C.García, J.Pérez, P.Callow, T.A.Ezquerra, and J.González-Rodríguez (2010).
Three-dimensional model of human platelet integrin alphaIIb beta3 in solution obtained by small angle neutron scattering.
  J Biol Chem, 285, 1023-1031.  
20584077 C.Rosano, and M.Rocco (2010).
Solution properties of full-length integrin alpha(IIb)beta3 refined models suggest environment-dependent induction of alternative bent /extended resting states.
  FEBS J, 277, 3190-3202.  
20033057 C.Xie, J.Zhu, X.Chen, L.Mi, N.Nishida, and T.A.Springer (2010).
Structure of an integrin with an alphaI domain, complement receptor type 4.
  EMBO J, 29, 666-679.
PDB codes: 3k6s 3k71 3k72
20679525 J.Zhu, J.Zhu, A.Negri, D.Provasi, M.Filizola, B.S.Coller, and T.A.Springer (2010).
Closed headpiece of integrin αIIbβ3 and its complex with an αIIbβ3-specific antagonist that does not induce opening.
  Blood, 116, 5050-5059.
PDB codes: 3nid 3nif 3nig
21134644 N.J.Anthis, K.L.Wegener, D.R.Critchley, and I.D.Campbell (2010).
Structural diversity in integrin/talin interactions.
  Structure, 18, 1654-1666.  
20308986 S.J.Shattil, C.Kim, and M.H.Ginsberg (2010).
The final steps of integrin activation: the end game.
  Nat Rev Mol Cell Biol, 11, 288-300.  
19917607 T.A.Bunch (2010).
Integrin alphaIIbbeta3 activation in Chinese hamster ovary cells and platelets increases clustering rather than affinity.
  J Biol Chem, 285, 1841-1849.  
  20168080 T.S.Ulmer (2010).
Structural basis of transmembrane domain interactions in integrin signaling.
  Cell Adh Migr, 4, 243-248.  
21098296 Y.Pan, K.Zhang, J.Qi, J.Yue, T.A.Springer, and J.Chen (2010).
Cation-pi interaction regulates ligand-binding affinity and signaling of integrin alpha4beta7.
  Proc Natl Acad Sci U S A, 107, 21388-21393.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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