PDBsum entry 3ihp

Hydrolase

PDB id: 3ihp

Contents

Protein chains

673 a.a. *

75 a.a. *

Ligands

NEH ×2

Metals

_ZN ×2

_CL

Waters ×32

* Residue conservation analysis

PDB id:

3ihp

Name:

Hydrolase

Title:

Covalent ubiquitin-usp5 complex

Structure:

Ubiquitin carboxyl-terminal hydrolase 5. Chain: a, b. Synonym: ubiquitin thioesterase 5, ubiquitin-specific-processing protease 5, deubiquitinating enzyme 5, isopeptidase t. Engineered: yes. Ubiquitin. Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: isot, usp5. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: rps27a, uba52, uba80, ubb, ubc, ubcep1, ubcep2.

Resolution:

2.80Å R-factor: 0.227 R-free: 0.276

Authors:

J.R.Walker,G.V.Avvakumov,S.Xue,C.Butler-Cole,J.Weigelt,C.Bountra, C.H.Arrowsmith,A.M.Edwards,A.Bochkarev,S.Dhe-Paganon,Structural Genomics Consortium (Sgc)

Key ref:

J.R.Walker et al. Covalent ubiquitin-Usp5 complex. To be published, .

Date:

30-Jul-09 Release date: 29-Dec-09

Protein chains

P45974  (UBP5_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 5 from Homo sapiens

Seq: 858 a.a.

Struc: 673 a.a.

Protein chains

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 75 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 3: Chains C, D: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.