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PDBsum entry 3i61
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Hydrolase/RNA
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PDB id
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3i61
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References listed in PDB file
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Key reference
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Title
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Structure of the yeast dead box protein mss116p reveals two wedges that crimp RNA.
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Authors
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M.Del campo,
A.M.Lambowitz.
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Ref.
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Mol Cell, 2009,
35,
598-609.
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PubMed id
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Abstract
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The yeast DEAD box protein Mss116p is a general RNA chaperone that functions in
mitochondrial group I and II intron splicing, translational activation, and RNA
end processing. Here we determined high-resolution X-ray crystal structures of
Mss116p complexed with an RNA oligonucleotide and ATP analogs AMP-PNP,
ADP-BeF(3)(-), or ADP-AlF(4)(-). The structures show the entire helicase core
acting together with a functionally important C-terminal extension. In all
structures, the helicase core is in a closed conformation with a wedge alpha
helix bending RNA 3' of the central bound nucleotides, as in previous DEAD box
protein structures. Notably, Mss116p's C-terminal extension also bends RNA 5' of
the central nucleotides, resulting in RNA crimping. Despite reported functional
differences, we observe few structural changes in ternary complexes with
different ATP analogs. The structures constrain models of DEAD box protein
function and reveal a strand separation mechanism in which a protein uses two
wedges to act as a molecular crimper.
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