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PDBsum entry 3i4m

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Transcription,transferase/DNA-RNA hybrid PDB id
3i4m
Jmol
Contents
Protein chains
1429 a.a.
1125 a.a.
270 a.a.
187 a.a.
214 a.a.
88 a.a.
171 a.a.
137 a.a.
116 a.a.
65 a.a.
116 a.a.
47 a.a.
DNA/RNA
Metals
_ZN ×8
_MG

References listed in PDB file
Key reference
Title Molecular basis of transcriptional mutagenesis at 8-Oxoguanine.
Authors G.E.Damsma, P.Cramer.
Ref. J Biol Chem, 2009, 284, 31658-31663.
PubMed id 19758983
DOI number 10.1074/JBC.M109.022764
Abstract
Structure-function analysis has revealed the mechanism of yeast RNA polymerase II transcription at 8-oxoguanine (8-oxoG), the major DNA lesion resulting from oxidative stress. When polymerase II encounters 8-oxoG in the DNA template strand, it can misincorporate adenine, which forms a Hoogsteen bp with 8-oxoG at the active center. This requires rotation of the 8-oxoG base from the standard anti- to an uncommon syn-conformation, which likely occurs during 8-oxoG loading into the active site. The misincorporated adenine escapes intrinsic proofreading, resulting in transcriptional mutagenesis that is observed directly by mass spectrometric RNA analysis.
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