PDBsum entry 3i2c

Immune system

PDB id: 3i2c

Contents

Protein chains

218 a.a. *

222 a.a. *

Ligands

GOL

Waters ×2

* Residue conservation analysis

PDB id:

3i2c

Name:

Immune system

Title:

Crystal structure of anti-il-23 antibody cnto4088

Structure:

Cnto4088 light chain. Chain: l. Engineered: yes. Cnto4088 heavy chain. Chain: h. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek 293. Expression_system_organ: kidney. Expression_system_cell: human embryonic kidney 293 cells. Expression_system_cell: human embryonic kidney 293 cells

Resolution:

2.80Å R-factor: 0.198 R-free: 0.235

Authors:

A.Teplyakov,G.Obmolova,G.L.Gilliland

Key ref:

A.Teplyakov et al. (2010). Noncanonical conformation of CDR L1 in the anti-IL-23 antibody CNTO4088. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 229-232. PubMed id: 20208149

Date:

29-Jun-09 Release date: 07-Jul-09

Protein chain

No UniProt id for this chain

Struc: 218 a.a.

Protein chain

No UniProt id for this chain

Struc: 222 a.a.

Acta Crystallogr Sect F Struct Biol Cryst Commun 66:229-232 (2010)

PubMed id: 20208149

Noncanonical conformation of CDR L1 in the anti-IL-23 antibody CNTO4088.

A.Teplyakov, G.Obmolova, A.Rogers, G.L.Gilliland.

ABSTRACT

CNTO4088 is a monoclonal antibody to human IL-23. The X-ray structure of the Fab fragment revealed an unusual noncanonical conformation of CDR L1. Most antibodies with the kappa light chain exhibit a canonical structure for CDR L1 in which residue 29 anchors the CDR loop to the framework. Analysis of the residues believed to define the conformation of CDR L1 did not explain why it should not adopt a canonical conformation in this antibody. This makes CNTO4088 a benchmark case for developing prediction methods and structure-modeling tools.