UniProt functional annotation for P34087



	UniProt functional annotation for P34087

UniProt code: P34087.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. {ECO:0000269|PubMed:11087726, ECO:0000269|PubMed:15304220, ECO:0000269|PubMed:17875743}.

Subunit: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex. The RPB4-RPB7 subcomplex probably associates with TFG1. Interacts with NRD1 and PAT1. {ECO:0000269|PubMed:12907709, ECO:0000269|PubMed:12914699, ECO:0000269|PubMed:14580350, ECO:0000269|PubMed:16341226, ECO:0000269|PubMed:17875743}.

Subcellular location: Nucleus. Cytoplasm. Cytoplasm, P-body. Note=Seems to shuttle between nucleus and cytoplasm in a complex with RPB4.

Miscellaneous: Present with 6420 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Similarity: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. {ECO:0000269\|PubMed:11087726, ECO:0000269\|PubMed:15304220, ECO:0000269\|PubMed:17875743}.


Subunit:		Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex. The RPB4-RPB7 subcomplex probably associates with TFG1. Interacts with NRD1 and PAT1. {ECO:0000269\|PubMed:12907709, ECO:0000269\|PubMed:12914699, ECO:0000269\|PubMed:14580350, ECO:0000269\|PubMed:16341226, ECO:0000269\|PubMed:17875743}.
Subcellular location:		Nucleus. Cytoplasm. Cytoplasm, P-body. Note=Seems to shuttle between nucleus and cytoplasm in a complex with RPB4.
Miscellaneous:		Present with 6420 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity:		Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. {ECO:0000305}.