UniProt functional annotation for Q6NS38



	UniProt functional annotation for Q6NS38

UniProt code: Q6NS38.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Dioxygenase that repairs alkylated DNA and RNA containing 1- methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single- stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:18432238, ECO:0000269|PubMed:18519673}.

Catalytic activity: Reaction=2-oxoglutarate + a methylated nucleobase within DNA + O2 = a nucleobase within DNA + CO2 + formaldehyde + succinate; Xref=Rhea:RHEA:30299, Rhea:RHEA-COMP:12192, Rhea:RHEA-COMP:12193, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:16842, ChEBI:CHEBI:30031, ChEBI:CHEBI:32875, ChEBI:CHEBI:64428; EC=1.14.11.33;

Cofactor: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000255|PROSITE-ProRule:PRU00805, ECO:0000269|PubMed:18432238, ECO:0000269|PubMed:18519673}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE- ProRule:PRU00805, ECO:0000269|PubMed:18432238, ECO:0000269|PubMed:18519673};

Activity regulation: Activated by ascorbate and magnesium ions. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:18519673}.

Subcellular location: Nucleus {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517}. Note=Detected in replication foci during S-phase.

Tissue specificity: Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:16174769}.

Similarity: Belongs to the alkB family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Dioxygenase that repairs alkylated DNA and RNA containing 1- methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single- stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. {ECO:0000269\|PubMed:12486230, ECO:0000269\|PubMed:12594517, ECO:0000269\|PubMed:16174769, ECO:0000269\|PubMed:18432238, ECO:0000269\|PubMed:18519673}.


Catalytic activity:		Reaction=2-oxoglutarate + a methylated nucleobase within DNA + O2 = a nucleobase within DNA + CO2 + formaldehyde + succinate; Xref=Rhea:RHEA:30299, Rhea:RHEA-COMP:12192, Rhea:RHEA-COMP:12193, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:16842, ChEBI:CHEBI:30031, ChEBI:CHEBI:32875, ChEBI:CHEBI:64428; EC=1.14.11.33;
Cofactor:		Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00805, ECO:0000269\|PubMed:18432238, ECO:0000269\|PubMed:18519673}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255\|PROSITE- ProRule:PRU00805, ECO:0000269\|PubMed:18432238, ECO:0000269\|PubMed:18519673};
Activity regulation:		Activated by ascorbate and magnesium ions. {ECO:0000269\|PubMed:12486230, ECO:0000269\|PubMed:18519673}.
Subcellular location:		Nucleus {ECO:0000269\|PubMed:12486230, ECO:0000269\|PubMed:12594517}. Note=Detected in replication foci during S-phase.
Tissue specificity:		Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. {ECO:0000269\|PubMed:12486230, ECO:0000269\|PubMed:16174769}.
Similarity:		Belongs to the alkB family. {ECO:0000305}.