PDBsum entry 3h1u

Signaling protein

PDB id: 3h1u

Contents

Protein chains

75 a.a. *

Metals

_CD ×5

Waters ×42

* Residue conservation analysis

PDB id:

3h1u

Name:

Signaling protein

Title:

Structure of ubiquitin in complex with cd ions

Structure:

Ubiquitin. Chain: a, b

Source:

Bos taurus. Bovine. Organism_taxid: 9913

Resolution:

3.00Å R-factor: 0.222 R-free: 0.257

Authors:

I.A.Qureshi,F.Ferron,P.Cheung,J.Lescar

Key ref:

I.A.Qureshi et al. (2009). Crystallographic structure of ubiquitin in complex with cadmium ions. Bmc Res Notes, 2, 251. PubMed id: 20003470

Date:

14-Apr-09 Release date: 05-May-09

Protein chains

P0CH28  (UBC_BOVIN) -  Polyubiquitin-C from Bos taurus

Seq: 690 a.a.

Struc: 75 a.a.

Enzyme reactions

• Enzyme class: E.C.?

Bmc Res Notes 2:251 (2009)

PubMed id: 20003470

Crystallographic structure of ubiquitin in complex with cadmium ions.

I.A.Qureshi, F.Ferron, C.C.Seh, P.Cheung, J.Lescar.

ABSTRACT

BACKGROUND: Ubiquitination plays a critical role in regulating many cellular processes, from DNA repair and gene transcription to cell cycle and apoptosis. It is catalyzed by a specific enzymatic cascade ultimately leading to the conjugation of ubiquitin to lysine residues of the target protein that can be the ubiquitin molecule itself and to the formation of poly-ubiquitin chains. FINDINGS: We present the crystal structure at 3.0 A resolution of bovine ubiquitin crystallized in presence of cadmium ions. Two molecules of ubiquitin are present in the asymmetric unit. Interestingly this non-covalent dimeric arrangement brings Lys-6 and Lys-63 of each crystallographically-independent monomer in close contact with the C-terminal ends of the other monomer. Residues Leu-8, Ile-44 and Val-70 that form a hydrophobic patch at the surface of the Ub monomer are trapped at the dimer interface. CONCLUSIONS: The structural basis for signalling by poly-Ub chains relies on a visualization of conformations of alternatively linked poly-Ub chains. This arrangement of ubiquitin could illustrate how linkages involving Lys-6 or Lys-63 of ubiquitin are produced in the cell. It also details how ubiquitin molecules can specifically chelate cadmium ions.