PDBsum entry 3gu4

Transferase

PDB id: 3gu4

Contents

Protein chain

280 a.a. *

Ligands

ANP

Waters ×338

* Residue conservation analysis

PDB id:

3gu4

Name:

Transferase

Title:

Crystal structure of dapkq23v-amppnp

Structure:

Death-associated protein kinase 1. Chain: a. Fragment: protein kinase domain. Synonym: dap kinase 1. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: dapk, dapk1, death-associated protein kinase 1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.35Å R-factor: 0.185 R-free: 0.211

Authors:

L.K.Mcnamara,J.S.Schavocky,D.M.Watterson,J.S.Brunzelle

Key ref:

L.K.Mcnamara et al. Enzymatic activity and crystallgoraphic analyses of a glycine-Rich loop mutant of dapk. To be published, .

Date:

28-Mar-09 Release date: 09-Mar-10

Protein chain

P53355  (DAPK1_HUMAN) -  Death-associated protein kinase 1 from Homo sapiens

Seq: 1430 a.a.

Struc: 280 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site