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UniProt functional annotation for P84131 | ||
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| UniProt code: P84131. |
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| Organism: | |
Geobacillus stearothermophilus (Bacillus stearothermophilus). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. |
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| Function: | |
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. {ECO:0000256|HAMAP-Rule:MF_00103}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine.; EC=3.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001668, ECO:0000256|HAMAP- Rule:MF_00103}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|ARBA:ARBA00001947}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00103}; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00103}; |
| Subunit: | |
Monomer. {ECO:0000256|ARBA:ARBA00011245, ECO:0000256|HAMAP- Rule:MF_00103}. |
| Similarity: | |
Belongs to the FPG family. {ECO:0000256|ARBA:ARBA00009409, ECO:0000256|HAMAP-Rule:MF_00103}. |
Annotations taken from UniProtKB at the EBI.