PDBsum entry 3gof

Metal binding protein/oxidoreductase

PDB id: 3gof

Contents

Protein chains

144 a.a. *

16 a.a. *

Ligands

SO4 ×2

Metals

_CA ×8

Waters ×511

* Residue conservation analysis

PDB id:

3gof

Name:

Metal binding protein/oxidoreductase

Title:

Calmodulin bound to peptide from macrophage nitric oxide synthase

Structure:

Calmodulin. Chain: a, b. Synonym: cam. Engineered: yes. Nitric oxide synthase, inducible. Chain: c, d. Fragment: calcium binding domain. Synonym: inducible no synthase, inducible nos, inos, nos type ii, macrophage nos, mac-nos.

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Gene: calm, cam, rcjmb04_24e7. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide is naturally found in mus musculus (mouse).

Resolution:

1.45Å R-factor: 0.148 R-free: 0.208

Authors:

H.L.Ng,A.Greenstein,M.Marletta,A.J.Wand,T.Alber

Key ref:

H.L.Ng et al. Structural diversity in calmodulin recognition of nitric oxide synthases. To be published, .

Date:

19-Mar-09 Release date: 31-Mar-10

Protein chains

P62149  (CALM_CHICK) -  Calmodulin from Gallus gallus

Seq: 149 a.a.

Struc: 144 a.a.

Protein chains

P29477  (NOS2_MOUSE) -  Nitric oxide synthase, inducible from Mus musculus

Seq: 1144 a.a.

Struc: 16 a.a.

Enzyme reactions

• Enzyme class: Chains C, D: E.C.1.14.13.39 - nitric-oxide synthase (NADPH).
• Reaction: 2 L-arginine + 3 NADPH + 4 O2 + H⁺ = 2 L-citrulline + 2 nitric oxide + 3 NADP⁺ + 4 H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site