PDBsum entry 3g8k

Immune system

PDB id

3g8k

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Contents

			Protein chains

					127 a.a. *

			Waters ×160

* Residue conservation analysis

PDB id:

3g8k

Links

Name:

Immune system

Title:

Crystal structure of murine natural killer cell receptor, ly49l4

Structure:

Lectin-related nk cell receptor ly49l1. Chain: a, b. Fragment: c-type lectin-like domain (unp residues 155-281). Engineered: yes. Mutation: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: klra12, mouse. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å

R-factor:

0.226

R-free:

0.288

Authors:

S.Cho

Key ref:

J.Back et al. (2009). Distinct conformations of Ly49 natural killer cell receptors mediate MHC class I recognition in trans and cis. Immunity, 31, 598-608. PubMed id: 19818651

Date:

12-Feb-09

Release date:

17-Nov-09

PROCHECK

	Headers

	References

Protein chains

Q9JIQ2 (Q9JIQ2_MOUSE) - Lectin-related NK cell receptor LY49L1 from Mus musculus

Seq: Struc:					281 a.a. 127 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Immunity 31:598-608 (2009)
PubMed id: 19818651

Distinct conformations of Ly49 natural killer cell receptors mediate MHC class I recognition in trans and cis.
J.Back, E.L.Malchiodi, S.Cho, L.Scarpellino, P.Schneider, M.C.Kerzic, R.A.Mariuzza, W.Held.

ABSTRACT

Certain cell-surface receptors engage ligands expressed on juxtaposed cells and ligands on the same cell. The structural basis for trans versus cis binding is not known. Here, we showed that Ly49 natural killer (NK) cell receptors bound two MHC class I (MHC-I) molecules in trans when the two ligand-binding domains were backfolded onto the long stalk region. In contrast, dissociation of the ligand-binding domains from the stalk and their reorientation relative to the NK cell membrane allowed monovalent binding of MHC-I in cis. The distinct conformations (backfolded and extended) define the structural basis for cis-trans binding by Ly49 receptors and explain the divergent functional consequences of cis versus trans interactions. Further analyses identified specific stalk segments that were not required for MHC-I binding in trans but were essential for inhibitory receptor function. These data identify multiple distinct roles of stalk regions for receptor function.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20976309

T.Takai, A.Nakamura, and S.Endo (2011).
Role of PIR-B in autoimmune glomerulonephritis.

J Biomed Biotechnol, 2011, 275302.

21159498

W.Held, M.Kijima, G.Angelov, and S.Bessoles (2011).
The function of natural killer cells: education, reminders and some good memories.

Curr Opin Immunol, 23, 228-233.

20952682

M.H.Brown, and E.Lacey (2010).
A ligand for CD5 is CD5.

J Immunol, 185, 6068-6074.

20957233

P.Brodin, T.Lakshmikanth, R.Mehr, M.H.Johansson, A.D.Duru, A.Achour, M.Salmon-Divon, K.Kärre, P.Höglund, and S.Johansson (2010).
Natural killer cell tolerance persists despite significant reduction of self MHC class I on normal target cells in mice.

PLoS One, 5, 0.

20818413

P.Höglund, and P.Brodin (2010).
Current perspectives of natural killer cell education by MHC class I molecules.

Nat Rev Immunol, 10, 724-734.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.