PDBsum entry 3fyh

Recombination

PDB id: 3fyh

Contents

Protein chain

296 a.a. *

Ligands

ADP

Metals

__W ×24

_MG ×2

_NA

Waters ×123

* Residue conservation analysis

PDB id:

3fyh

Name:

Recombination

Title:

Recombinase in complex with adp and metatungstate

Structure:

DNA repair and recombination protein rada. Chain: a. Engineered: yes. Mutation: yes

Source:

Methanococcus voltae. Organism_taxid: 2188. Gene: rada. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.200 R-free: 0.227

Authors:

Y.Li,Y.He,Y.Luo

Key ref:

Y.Li et al. (2009). Crystal structure of an archaeal Rad51 homologue in complex with a metatungstate inhibitor. Biochemistry, 48, 6805-6810. PubMed id: 19555119

Date:

22-Jan-09 Release date: 01-Sep-09

Protein chain

O73948  (RADA_METVO) -  DNA repair and recombination protein RadA from Methanococcus voltae

Seq: 322 a.a.

Struc: 296 a.a.

Biochemistry 48:6805-6810 (2009)

PubMed id: 19555119

Crystal structure of an archaeal Rad51 homologue in complex with a metatungstate inhibitor.

Y.Li, Y.He, Y.Luo.

ABSTRACT

Archaeal RadAs are close homologues of eukaryal Rad51s ( approximately 40% sequence identities). These recombinases promote a hallmark strand exchange process between homologous single-stranded and double-stranded DNA substrates. This DNA-repairing function also plays a key role in cancer cells' resistance to chemo- and radiotherapy. Inhibition of the strand exchange process may render cancer cells more susceptible to therapeutic treatment. We found that metatungstate is a potent inhibitor of RadA from Methanococcus voltae. The tungsten cluster binds RadA in the axial DNA-binding groove. This polyanionic species appears to inhibit RadA by locking the protein in its inactive conformation.