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UniProt functional annotation for P13382 | ||
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| UniProt code: P13382. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalytic component of DNA polymerase alpha, which in complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase (PubMed:10898792, PubMed:22119860, PubMed:31488849). POL1 has a role in promoting telomere replication during interaction with CDC13 (PubMed:10898792). {ECO:0000269|PubMed:10898792, ECO:0000269|PubMed:22119860, ECO:0000269|PubMed:31488849}. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; Evidence={ECO:0000269|PubMed:22119860}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:22119860}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000269|PubMed:22119860}; |
| Subunit: | |
DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A (By similarity). POL1 interacts with CDC13, POB3, SPT16 and MCM10. {ECO:0000250, ECO:0000269|PubMed:10898792, ECO:0000269|PubMed:16675460, ECO:0000269|PubMed:8621497, ECO:0000269|PubMed:9199353}. |
| Subcellular location: | |
Nucleus. |
| Domain: | |
The CysA-type zinc finger is required for PCNA-binding. {ECO:0000250|UniProtKB:P15436}. |
| Domain: | |
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes. {ECO:0000250|UniProtKB:P15436}. |
| Miscellaneous: | |
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis. |
| Miscellaneous: | |
Present with 1050 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the DNA polymerase type-B family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.