PDBsum entry 3fl2

Ligase

PDB id: 3fl2

Contents

Protein chain

116 a.a. *

Metals

_ZN ×2

Waters ×100

* Residue conservation analysis

PDB id:

3fl2

Name:

Ligase

Title:

Crystal structure of the ring domain of the e3 ubiquitin-protein ligase uhrf1

Structure:

E3 ubiquitin-protein ligase uhrf1. Chain: a. Fragment: ring domain (unp residues 672-793). Synonym: ubiquitin-like phd and ring finger domain-containing protein 1, ubiquitin-like-containing phd and ring finger domains protein 1, inverted ccaat box-binding protein of 90 kda, transcription factor icbp90, nuclear zinc finger protein np95, nuclear protein 95, hunp95, ring finger protein 106. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: icbp90, np95, rnf106, uhrf1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å R-factor: 0.187 R-free: 0.250

Authors:

J.R.Walker,G.V.Avvakumov,S.Xue,Y.Li,C.Bountra,J.Weigelt, C.H.Arrowsmith,A.M.Edwards,A.Bochkarev,S.Dhe-Paganon,Structural Genomics Consortium (Sgc)

Key ref:

J.R.Walker et al. Structure of the ring domain of the e3 ubiquitin-Protein ligase uhrf1. To be published, .

Date:

18-Dec-08 Release date: 20-Jan-09

Protein chain

Q96T88  (UHRF1_HUMAN) -  E3 ubiquitin-protein ligase UHRF1 from Homo sapiens

Seq: 793 a.a.

Struc: 116 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine