PDBsum entry 3fe3

Transferase

PDB id: 3fe3

Contents

Protein chains

317 a.a. *

Waters ×398

* Residue conservation analysis

PDB id:

3fe3

Name:

Transferase

Title:

Crystal structure of the kinase mark3/par-1: t211a-s215a double mutant

Structure:

Map/microtubule affinity-regulating kinase 3. Chain: a, b. Fragment: catalytic and ubiquitin-associated domains, unp residues 41-367. Synonym: cdc25c-associated protein kinase 1, c-tak1, ctak1, serine/threonine protein kinase p78, ser/thr protein kinase par-1, protein kinase stk10. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mark3. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.199 R-free: 0.233

Authors:

C.Nugoor,A.Marx,S.Panneerselvam,E.-M.Mandelkow,E.Mandelkow

Key ref:

C.Nugoor et al. Crystal structure of the kinase mark3/par-1: t211a-S215a double mutant. To be published, .

Date:

27-Nov-08 Release date: 16-Dec-08

Protein chains

P27448  (MARK3_HUMAN) -  MAP/microtubule affinity-regulating kinase 3 from Homo sapiens

Seq: 753 a.a.

Struc: 317 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site