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PDBsum entry 3fas
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Membrane protein
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PDB id
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3fas
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Contents |
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* Residue conservation analysis
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PDB id:
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Membrane protein
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Title:
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X-ray structure of iglur4 flip ligand-binding core (s1s2) in complex with (s)-glutamate at 1.40a resolution
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Structure:
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Glutamate receptor 4. Chain: a, b. Fragment: iglur4 flip ligand-binding core (s1s2). Synonym: ionotropic glutamate receptor 4, glur-4, glur4, glur-d, glutamate receptor ionotropic, ampa 4, ampa-selective glutamate receptor 4. Engineered: yes
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Source:
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Rattus norvegicus. Rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.40Å
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R-factor:
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0.167
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R-free:
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0.183
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Authors:
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C.Kasper,K.Frydenvang,P.Naur,M.Gajhede,J.S.Kastrup
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Key ref:
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C.Kasper
et al.
(2008).
Molecular mechanism of agonist recognition by the ligand-binding core of the ionotropic glutamate receptor 4.
Febs Lett,
582,
4089-4094.
PubMed id:
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Date:
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18-Nov-08
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Release date:
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09-Dec-08
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PROCHECK
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Headers
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References
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P19493
(GRIA4_RAT) -
Glutamate receptor 4 from Rattus norvegicus
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Seq:
Struc:
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902 a.a.
260 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 10 residue positions (black
crosses)
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Febs Lett
582:4089-4094
(2008)
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PubMed id:
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Molecular mechanism of agonist recognition by the ligand-binding core of the ionotropic glutamate receptor 4.
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C.Kasper,
K.Frydenvang,
P.Naur,
M.Gajhede,
D.S.Pickering,
J.S.Kastrup.
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ABSTRACT
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The alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) class of
ionotropic glutamate receptors comprises four different subunits: iGluR1/iGluR2
and iGluR3/iGluR4 forming two subgroups. Three-dimensional structures have been
reported only of the ligand-binding core of iGluR2. Here, we present two X-ray
structures of a soluble construct of the R/G unedited flip splice variant of the
ligand-binding core of iGluR4 (iGluR4(i)(R)-S1S2) in complex with glutamate or
AMPA. Subtle, but important differences are found in the ligand-binding cavity
between the two AMPA receptor subgroups at position 724 (Tyr in iGluR1/iGluR2
and Phe in iGluR3/iGluR4), which in iGluR4 may lead to displacement of a water
molecule and hence points to the possibility to make subgroup specific ligands.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.Pøhlsgaard,
K.Frydenvang,
U.Madsen,
and
J.S.Kastrup
(2011).
Lessons from more than 80 structures of the GluA2 ligand-binding domain in complex with agonists, antagonists and allosteric modulators.
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Neuropharmacology,
60,
135-150.
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M.L.Mayer
(2011).
Structure and mechanism of glutamate receptor ion channel assembly, activation and modulation.
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Curr Opin Neurobiol,
21,
283-290.
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R.Vijayan,
M.A.Sahai,
T.Czajkowski,
and
P.C.Biggin
(2010).
A comparative analysis of the role of water in the binding pockets of ionotropic glutamate receptors.
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Phys Chem Chem Phys,
12,
14057-14066.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
