PDBsum entry 3f73

Nucleic acid binding protein/DNA/RNA

PDB id

3f73

Contents

			Protein chains

					678 a.a.

			DNA/RNA

			Ligands

					PO4

			Metals

					_MG ×3

			Waters ×16

References listed in PDB file

Key reference

Title

Structure of an argonaute silencing complex with a seed-Containing guide DNA and target RNA duplex.

Authors

Y.Wang, S.Juranek, H.Li, G.Sheng, T.Tuschl, D.J.Patel.

Ref.

Nature, 2008, 456, 921-926. [DOI no: 10.1038/nature07666]

PubMed id

19092929

Note: In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above have been manually determined.

Abstract

Here we report on a 3.0 A crystal structure of a ternary complex of wild-type Thermus thermophilus argonaute bound to a 5'-phosphorylated 21-nucleotide guide DNA and a 20-nucleotide target RNA containing cleavage-preventing mismatches at the 10-11 step. The seed segment (positions 2 to 8) adopts an A-helical-like Watson-Crick paired duplex, with both ends of the guide strand anchored in the complex. An arginine, inserted between guide-strand bases 10 and 11 in the binary complex, locking it in an inactive conformation, is released on ternary complex formation. The nucleic-acid-binding channel between the PAZ- and PIWI-containing lobes of argonaute widens on formation of a more open ternary complex. The relationship of structure to function was established by determining cleavage activity of ternary complexes containing position-dependent base mismatch, bulge and 2'-O-methyl modifications. Consistent with the geometry of the ternary complex, bulges residing in the seed segments of the target, but not the guide strand, were better accommodated and their complexes were catalytically active.



	Figure 1. Figure 1: Crystal structure of T. thermophilus Ago bound to 5'-phosphorylated 21-nucleotide guide DNA and 20-nucleotide target RNA. a, Sequence of the guide DNA-target RNA duplex. The traceable segments of the bases of the guide DNA and target RNA in the structure of the ternary complex are shown in red and blue, respectively. Disordered segments of the bases on both strands that cannot be traced are shown in grey. b, Stereo view of the 3.0 Å crystal structure of the Ago ternary complex. The Ago protein is colour-coded by domains (N in cyan, PAZ in magenta, Mid in orange and PIWI in green) and linkers (L1 and L2 in grey). The bound 21-nucleotide guide DNA is in red and traced for bases of the 1–10 and 19–21 segments, whereas the bound 20-nucleotide target RNA is in blue and traced for bases of the 1' to 9' segment. Backbone phosphorus atoms are in yellow. c, An alternate view of the complex.		Figure 2. Figure 2: Comparison of structural details between the binary Ago complex with bound guide DNA and the ternary complex with added target RNA. a, Expanded view of the ternary complex highlighting the guide DNA (1–10)-target RNA (1'–9') duplex and Mg^2+-coordinated catalytic residues (D478, D546 and D660) of the RNase H fold of the PIWI domain. Intermolecular hydrogen bonds between the Ago protein and the DNA guide strand in red are shown by dashed lines. b, Positioning of the sugar-phosphate backbone of the target RNA strand spanning the mismatch-containing 10–11-step relative to the catalytic residues of the PIWI domain. c, Comparison of the trajectory of traceable bound guide DNA in the binary (bases 1–11 and 18–21 in silver) and ternary (bases 1–10 and 19–21 in red) Ago complexes after superposition of their 5'-phosphate-binding pockets. d, Superposition of the guide DNA (red)-target RNA (blue) duplex spanning the 2–8 seed segment on A-form (left panel) and B-form (right panel) helices (silver) after best-fit superposition of the target RNA strand of the ternary Ago complex with one strand of the A/B-form helices. e, Positioning of stacked residues 6–10 of the DNA guide strand relative to R548, with emphasis on intermolecular interactions involving the sugar-phosphate backbone. f, Relative positioning of the 6 to 10/11 segment of the bound guide DNA strand and R548 in the binary (guide strand in silver, protein in cyan) and ternary (guide strand in red, protein in magenta) Ago complexes. The conformational change in the protein on proceeding from binary to ternary Ago complexes is indicated by a red arrow.

PROCHECK

	Headers