UniProt functional annotation for P53615

UniProt code: P53615.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
 
Function: Catalyzes the reversible hydration of CO(2) to H(2)CO(3). The main role may be to provide inorganic carbon for the bicarbonate-dependent carboxylation reactions catalyzed by pyruvate carboxylase, acetyl-CoA carboxylase and carbamoyl- phosphate synthetase. Involved in protection against oxidative damage. Encodes a substrate for the non-classical protein export pathway for proteins that lack a cleavable signal sequence. {ECO:0000269|PubMed:10407265, ECO:0000269|PubMed:15096093, ECO:0000269|PubMed:15813743, ECO:0000269|PubMed:15948716, ECO:0000269|PubMed:18993072}.
 
Catalytic activity: H(2)CO(3) = CO(2) + H(2)O. {ECO:0000269|PubMed:15813743, ECO:0000269|PubMed:18993072}.
Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:19852838}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:19852838};
Biophysicochemical properties: Kinetic parameters: Note=kcat is 940000 sec(-1) with CO(2) as substrate. {ECO:0000269|PubMed:18993072};
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. Mitochondrion intermembrane space {ECO:0000269|PubMed:22984289}.
Induction: Transcription and activity down-regulated at elevated CO(2) concentrations. {ECO:0000269|PubMed:15780657, ECO:0000269|PubMed:15813743}.
Miscellaneous: Present with 2330 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity: Belongs to the beta-class carbonic anhydrase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.