|
|
||
|
|
|
|
|
UniProt functional annotation for Q24451 | ||
|
|
|
|
|
|
||
| UniProt code: Q24451. |
|
||
| Organism: | |
Drosophila melanogaster (Fruit fly). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. |
|
||
|
||
| Function: | |
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N- glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity). {ECO:0000250}. |
|
||
| Catalytic activity: | |
Reaction=2 H2O + N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha- D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] = 2 alpha-D-mannopyranose + N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc}-L-asparaginyl-[protein]; Xref=Rhea:RHEA:56052, Rhea:RHEA-COMP:14368, Rhea:RHEA-COMP:14369, ChEBI:CHEBI:15377, ChEBI:CHEBI:28729, ChEBI:CHEBI:60615, ChEBI:CHEBI:60625; EC=3.2.1.114; Evidence={ECO:0000269|PubMed:10504337}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Binds 1 zinc ion per subunit. {ECO:0000250}; |
| Pathway: | |
Protein modification; protein glycosylation. |
| Subunit: | |
Homodimer; disulfide-linked. {ECO:0000250}. |
| Subcellular location: | |
Golgi apparatus membrane {ECO:0000269|PubMed:10504337}; Single-pass type II membrane protein {ECO:0000269|PubMed:10504337}. |
| Induction: | |
Inhibited by swainsonine and by copper sulfate. {ECO:0000269|PubMed:10504337}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 38 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.