UniProt functional annotation for Q06142



	UniProt functional annotation for Q06142

UniProt code: Q06142.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485). At the nucleoplasmic side of the NPC, GTP- Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302). Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015). Mediates the nuclear import of histones H2A and H2B (PubMed:11309407). Mediates the nuclear import of transcription factor GCN4 (PubMed:14648200). {ECO:0000269|PubMed:11309407, ECO:0000269|PubMed:14648200, ECO:0000269|PubMed:15864302, ECO:0000269|PubMed:7622450, ECO:0000269|PubMed:8521485, ECO:0000305|PubMed:11423015}.

Subunit: Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450). Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403). Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174). {ECO:0000269|PubMed:15878174, ECO:0000269|PubMed:7622450, ECO:0000269|PubMed:8521485, ECO:0000269|PubMed:8621381, ECO:0000269|PubMed:9321403}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:10684247, ECO:0000269|PubMed:9321403}. Nucleus {ECO:0000269|PubMed:9321403}. Nucleus, nuclear pore complex {ECO:0000269|PubMed:10684247}.

Miscellaneous: Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site. {ECO:0000269|PubMed:8521485}.

Miscellaneous: The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP. {ECO:0000269|PubMed:8621381}.

Miscellaneous: Present with 51700 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Similarity: Belongs to the importin beta family. Importin beta-1 subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485). At the nucleoplasmic side of the NPC, GTP- Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302). Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015). Mediates the nuclear import of histones H2A and H2B (PubMed:11309407). Mediates the nuclear import of transcription factor GCN4 (PubMed:14648200). {ECO:0000269\|PubMed:11309407, ECO:0000269\|PubMed:14648200, ECO:0000269\|PubMed:15864302, ECO:0000269\|PubMed:7622450, ECO:0000269\|PubMed:8521485, ECO:0000305\|PubMed:11423015}.


Subunit:		Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450). Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403). Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174). {ECO:0000269\|PubMed:15878174, ECO:0000269\|PubMed:7622450, ECO:0000269\|PubMed:8521485, ECO:0000269\|PubMed:8621381, ECO:0000269\|PubMed:9321403}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:10684247, ECO:0000269\|PubMed:9321403}. Nucleus {ECO:0000269\|PubMed:9321403}. Nucleus, nuclear pore complex {ECO:0000269\|PubMed:10684247}.
Miscellaneous:		Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site. {ECO:0000269\|PubMed:8521485}.
Miscellaneous:		The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP. {ECO:0000269\|PubMed:8621381}.
Miscellaneous:		Present with 51700 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity:		Belongs to the importin beta family. Importin beta-1 subfamily. {ECO:0000305}.