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PDBsum entry 3e5x
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Transcription regulation
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PDB id
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3e5x
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References listed in PDB file
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Key reference
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Title
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Molecular basis of halorespiration control by cprk, A crp-Fnr type transcriptional regulator.
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Authors
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C.Levy,
K.Pike,
D.J.Heyes,
M.G.Joyce,
K.Gabor,
H.Smidt,
J.Van der oost,
D.Leys.
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Ref.
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Mol Microbiol, 2008,
70,
151-167.
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PubMed id
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Abstract
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Certain bacteria are able to conserve energy via the reductive dehalogenation of
halo-organic compounds in a respiration-type metabolism. The transcriptional
regulator CprK from Desulfitobacterium spp. induces expression of
halorespiratory genes upon binding of o-chlorophenol ligands and is reversibly
inactivated by oxygen through disulphide bond formation. We report crystal
structures of D. hafniense CprK in the ligand-free (both oxidation states),
ligand-bound (reduced) and DNA-bound states, making it the first member of the
widespread CRP-FNR superfamily for which a complete structural description of
both redox-dependent and allosteric molecular rearrangements is available. In
conjunction with kinetic and thermodynamic ligand binding studies, we provide a
model for the allosteric mechanisms underpinning transcriptional control. Amino
acids that play a key role in this mechanism are not conserved in functionally
distinct CRP-FNR members. This suggests that, despite significant structural
homology, distinct allosteric mechanisms are used, enabling this protein family
to control a very wide range of processes.
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