 |
PDBsum entry 3e4u
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Transcription
|
PDB id
|
|
|
|
3e4u
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
119 a.a.
|
|
|
|
|
|
|
|
|
|
|
120 a.a.
|
|
|
|
|
|
|
|
|
|
|
112 a.a.
|
|
|
|
|
|
|
|
|
|
|
110 a.a.
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Transcription
|
|
|
Title:
|
|
Crystal structure of the wild-type human bcl6 btb/poz domain
|
|
Structure:
|
|
B-cell lymphoma 6 protein. Chain: a, b, c, d, e, f. Fragment: btb domain. Synonym: bcl-6, zinc finger protein 51, laz-3 protein, bcl-5, zinc finger and btb domain-containing protein 27. Engineered: yes
|
|
Source:
|
|
Homo sapiens. Human. Organism_taxid: 9606. Gene: bcl6, bcl5, laz3, zbtb27, znf51. Expressed in: escherichia coli. Expression_system_taxid: 562.
|
|
Resolution:
|
|
|
2.10Å
|
R-factor:
|
0.221
|
R-free:
|
0.259
|
|
|
Authors:
|
|
M.A.Stead,G.O.Rosbrook,J.M.Hadden,C.H.Trinh,S.B.Carr,S.C.Wright
|
|
Key ref:
|
|
M.A.Stead
et al.
(2008).
Structure of the wild-type human BCL6 POZ domain.
Acta Crystallogr Sect F Struct Biol Cryst Commun,
64,
1101-1104.
PubMed id:
|
|
|
Date:
|
|
|
12-Aug-08
|
Release date:
|
09-Dec-08
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P41182
(BCL6_HUMAN) -
B-cell lymphoma 6 protein from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
706 a.a.
119 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
P41182
(BCL6_HUMAN) -
B-cell lymphoma 6 protein from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
706 a.a.
120 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
Chains A, B, C, D, E, F:
E.C.?
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Acta Crystallogr Sect F Struct Biol Cryst Commun
64:1101-1104
(2008)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Structure of the wild-type human BCL6 POZ domain.
|
|
M.A.Stead,
G.O.Rosbrook,
J.M.Hadden,
C.H.Trinh,
S.B.Carr,
S.C.Wright.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
BCL6 is a transcriptional repressor that is overexpressed in diffuse large
B-cell lymphoma and follicular lymphoma. The N-terminal POZ domain of BCL6
interacts with transcriptional corepressors and targeting these associations is
a promising therapeutic strategy. Previous structural studies of the BCL6 POZ
domain have used a mutant form because of the low solubility of the wild-type
recombinant protein. A method for the purification and crystallization of the
wild-type BCL6 POZ domain is described and the crystal structure to 2.1 A
resolution is reported. This will be relevant for the design of therapeutics
that target BCL6 POZ-domain interaction interfaces.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
| | |
Links
