spacer
spacer

PDBsum entry 3e1w

Go to PDB code: 
Top Page protein metals Protein-protein interface(s) links
Lyase PDB id
3e1w
Jmol
Contents
Protein chains
182 a.a.
Metals
_ZN ×2
Waters ×13
HEADER    LYASE                                   04-AUG-08   3E1W
TITLE     H. INFLUENZAE BETA-CARBONIC ANHYDRASE, VARIANT D44N IN 100 MM SODIUM
TITLE    2 BICARBONATE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CARBONIC ANHYDRASE 2;
COMPND   3 CHAIN: A, B;
COMPND   4 SYNONYM: CARBONATE DEHYDRATASE 2;
COMPND   5 EC: 4.2.1.1;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HAEMOPHILUS INFLUENZAE;
SOURCE   3 ORGANISM_TAXID: 727;
SOURCE   4 GENE: CAN, HI1301;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: JM109;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PTRC99
KEYWDS    BETA-CARBONIC ANHYDRASE, ACTIVE SITE MUTANT, LYASE, METAL-BINDING
EXPDTA    X-RAY DIFFRACTION
AUTHOR    R.S.ROWLETT
REVDAT   4   13-JUL-11 3E1W    1       VERSN
REVDAT   3   14-JUL-09 3E1W    1       JRNL
REVDAT   2   09-JUN-09 3E1W    1       REMARK
REVDAT   1   02-JUN-09 3E1W    0
JRNL        AUTH   R.S.ROWLETT,C.TU,J.LEE,A.G.HERMAN,D.A.CHAPNICK,S.H.SHAH,
JRNL        AUTH 2 P.C.GAREISS
JRNL        TITL   ALLOSTERIC SITE VARIANTS OF HAEMOPHILUS INFLUENZAE
JRNL        TITL 2 BETA-CARBONIC ANHYDRASE.
JRNL        REF    BIOCHEMISTRY                  V.  48  6146 2009
JRNL        REFN                   ISSN 0006-2960
JRNL        PMID   19459702
JRNL        DOI    10.1021/BI900663H
REMARK   2
REMARK   2 RESOLUTION.    2.60 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.2.0019
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.60
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.72
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.5
REMARK   3   NUMBER OF REFLECTIONS             : 18161
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.216
REMARK   3   R VALUE            (WORKING SET) : 0.215
REMARK   3   FREE R VALUE                     : 0.239
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100
REMARK   3   FREE R VALUE TEST SET COUNT      : 929
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.60
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.67
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1187
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 94.62
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2710
REMARK   3   BIN FREE R VALUE SET COUNT          : 61
REMARK   3   BIN FREE R VALUE                    : 0.3090
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2890
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 2
REMARK   3   SOLVENT ATOMS            : 13
REMARK   3
REMARK   3  B VALUES.
REMARK   3   B VALUE TYPE : LIKELY RESIDUAL
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 63.13
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.04000
REMARK   3    B22 (A**2) : 0.04000
REMARK   3    B33 (A**2) : -0.09000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.409
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.260
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.186
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 18.794
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.944
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.923
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  2960 ; 0.014 ; 0.021
REMARK   3   BOND LENGTHS OTHERS               (A):  1954 ; 0.002 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  4014 ; 1.457 ; 1.930
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  4758 ; 0.866 ; 3.002
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   362 ; 7.280 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   136 ;40.957 ;23.824
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   508 ;19.161 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    18 ;18.693 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   456 ; 0.071 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  3268 ; 0.004 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):   598 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1069 ; 0.253 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  2501 ; 0.224 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  1565 ; 0.203 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  1723 ; 0.096 ; 0.200
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   171 ; 0.161 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):     1 ; 0.049 ; 0.200
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    54 ; 0.216 ; 0.200
REMARK   3   SYMMETRY VDW OTHERS               (A):    63 ; 0.300 ; 0.200
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):     6 ; 0.205 ; 0.200
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  2236 ; 0.816 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):   745 ; 0.099 ; 1.500
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  2904 ; 1.000 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1326 ; 1.234 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1110 ; 1.856 ; 4.500
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 2
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A    34        A   215
REMARK   3    ORIGIN FOR THE GROUP (A):  62.6440  51.3870 165.7070
REMARK   3    T TENSOR
REMARK   3      T11:   0.0542 T22:  -0.1004
REMARK   3      T33:  -0.0891 T12:   0.0296
REMARK   3      T13:   0.0772 T23:   0.0465
REMARK   3    L TENSOR
REMARK   3      L11:   3.3012 L22:   1.9839
REMARK   3      L33:   2.2724 L12:  -0.4901
REMARK   3      L13:  -0.7619 L23:  -0.5313
REMARK   3    S TENSOR
REMARK   3      S11:   0.3100 S12:   0.3203 S13:   0.0495
REMARK   3      S21:  -0.4815 S22:  -0.2445 S23:  -0.1938
REMARK   3      S31:   0.2738 S32:   0.0665 S33:  -0.0654
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B    34        B   215
REMARK   3    ORIGIN FOR THE GROUP (A):  65.1430  41.0280 188.4460
REMARK   3    T TENSOR
REMARK   3      T11:  -0.0434 T22:  -0.1136
REMARK   3      T33:   0.0003 T12:  -0.0084
REMARK   3      T13:  -0.0394 T23:   0.0728
REMARK   3    L TENSOR
REMARK   3      L11:   2.4277 L22:   3.1029
REMARK   3      L33:   2.5609 L12:   0.4516
REMARK   3      L13:   0.2703 L23:  -0.8507
REMARK   3    S TENSOR
REMARK   3      S11:   0.1656 S12:  -0.2385 S13:  -0.2910
REMARK   3      S21:  -0.0086 S22:  -0.0751 S23:  -0.2966
REMARK   3      S31:   0.4162 S32:   0.2165 S33:  -0.0905
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : BABINET MODEL WITH MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK   3  POSITIONS
REMARK   4
REMARK   4 3E1W COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 15-AUG-08.
REMARK 100 THE RCSB ID CODE IS RCSB048770.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 11-APR-07
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 6.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : CHESS
REMARK 200  BEAMLINE                       : F2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.950
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 18278
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.600
REMARK 200  RESOLUTION RANGE LOW       (A) : 72.257
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.3
REMARK 200  DATA REDUNDANCY                : 9.700
REMARK 200  R MERGE                    (I) : 0.10800
REMARK 200  R SYM                      (I) : 0.10800
REMARK 200   FOR THE DATA SET  : 23.5000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.60
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.74
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.40
REMARK 200  R MERGE FOR SHELL          (I) : 0.53800
REMARK 200  R SYM FOR SHELL            (I) : 0.53800
REMARK 200   FOR SHELL         : 1.400
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 2A8D
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 53.84
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.66
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M MES, 0.8 M SODIUM ACETATE, 100
REMARK 280  MM SODIUM BICARBONATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP,
REMARK 280  TEMPERATURE 295K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 41 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z+1/2
REMARK 290       3555   -Y+1/2,X+1/2,Z+1/4
REMARK 290       4555   Y+1/2,-X+1/2,Z+3/4
REMARK 290       5555   -X+1/2,Y+1/2,-Z+1/4
REMARK 290       6555   X+1/2,-Y+1/2,-Z+3/4
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,-Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       91.05200
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       39.22500
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       39.22500
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       45.52600
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       39.22500
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       39.22500
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      136.57800
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       39.22500
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       39.22500
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       45.52600
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       39.22500
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       39.22500
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000      136.57800
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       91.05200
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 10060 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 25270 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -58.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT2   2  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      364.20800
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2890 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 14770 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -20.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     ASP A     2
REMARK 465     LYS A     3
REMARK 465     ILE A     4
REMARK 465     LYS A     5
REMARK 465     GLN A     6
REMARK 465     LEU A     7
REMARK 465     PHE A     8
REMARK 465     ALA A     9
REMARK 465     ASN A    10
REMARK 465     ASN A    11
REMARK 465     TYR A    12
REMARK 465     SER A    13
REMARK 465     TRP A    14
REMARK 465     ALA A    15
REMARK 465     GLN A    16
REMARK 465     ARG A    17
REMARK 465     MET A    18
REMARK 465     LYS A    19
REMARK 465     GLU A    20
REMARK 465     GLU A    21
REMARK 465     ASN A    22
REMARK 465     SER A    23
REMARK 465     THR A    24
REMARK 465     TYR A    25
REMARK 465     PHE A    26
REMARK 465     LYS A    27
REMARK 465     GLU A    28
REMARK 465     LEU A    29
REMARK 465     ALA A    30
REMARK 465     ASP A    31
REMARK 465     HIS A    32
REMARK 465     GLN A    33
REMARK 465     ASP A   216
REMARK 465     GLU A   217
REMARK 465     GLU A   218
REMARK 465     ASN A   219
REMARK 465     ILE A   220
REMARK 465     LEU A   221
REMARK 465     LYS A   222
REMARK 465     LYS A   223
REMARK 465     ASP A   224
REMARK 465     HIS A   225
REMARK 465     LEU A   226
REMARK 465     GLU A   227
REMARK 465     ASN A   228
REMARK 465     THR A   229
REMARK 465     MET B     1
REMARK 465     ASP B     2
REMARK 465     LYS B     3
REMARK 465     ILE B     4
REMARK 465     LYS B     5
REMARK 465     GLN B     6
REMARK 465     LEU B     7
REMARK 465     PHE B     8
REMARK 465     ALA B     9
REMARK 465     ASN B    10
REMARK 465     ASN B    11
REMARK 465     TYR B    12
REMARK 465     SER B    13
REMARK 465     TRP B    14
REMARK 465     ALA B    15
REMARK 465     GLN B    16
REMARK 465     ARG B    17
REMARK 465     MET B    18
REMARK 465     LYS B    19
REMARK 465     GLU B    20
REMARK 465     GLU B    21
REMARK 465     ASN B    22
REMARK 465     SER B    23
REMARK 465     THR B    24
REMARK 465     TYR B    25
REMARK 465     PHE B    26
REMARK 465     LYS B    27
REMARK 465     GLU B    28
REMARK 465     LEU B    29
REMARK 465     ALA B    30
REMARK 465     ASP B    31
REMARK 465     HIS B    32
REMARK 465     GLN B    33
REMARK 465     ASP B   216
REMARK 465     GLU B   217
REMARK 465     GLU B   218
REMARK 465     ASN B   219
REMARK 465     ILE B   220
REMARK 465     LEU B   221
REMARK 465     LYS B   222
REMARK 465     LYS B   223
REMARK 465     ASP B   224
REMARK 465     HIS B   225
REMARK 465     LEU B   226
REMARK 465     GLU B   227
REMARK 465     ASN B   228
REMARK 465     THR B   229
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    LEU A 174      117.56   -162.13
REMARK 500    ASP A 185        0.41   -157.11
REMARK 500    LEU A 188        3.59    -59.67
REMARK 500    VAL A 189       10.08     51.90
REMARK 500    ASP A 190       21.02    -64.81
REMARK 500    GLN A 191      -53.35    -11.06
REMARK 500    VAL A 193       92.04     85.69
REMARK 500    ASN B  44      106.18    -44.45
REMARK 500    VAL B  47       78.75   -118.30
REMARK 500    LEU B 115      -30.46    -39.63
REMARK 500    PRO B 139       40.14    -60.44
REMARK 500    GLU B 140      -49.16   -148.57
REMARK 500    ASP B 185     -166.06   -176.71
REMARK 500    PHE B 187      112.34    153.24
REMARK 500    VAL B 189       17.88    -54.91
REMARK 500    ASP B 190       99.94   -169.78
REMARK 500    MET B 194      114.35   -165.60
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500                                 MODEL     OMEGA
REMARK 500 ASP B  190     GLN B  191                  129.04
REMARK 500
REMARK 500 REMARK: NULL
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              ZN A 230  ZN
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 CYS A  42   SG
REMARK 620 2 HIS A  98   NE2 102.5
REMARK 620 3 CYS A 101   SG  117.2 110.7
REMARK 620 N                    1     2
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              ZN B 230  ZN
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 CYS B  42   SG
REMARK 620 2 HIS B  98   NE2 106.8
REMARK 620 3 CYS B 101   SG  116.2 107.9
REMARK 620 N                    1     2
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN A 230
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN B 230
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 2A8C   RELATED DB: PDB
REMARK 900 RELATED ID: 2A8D   RELATED DB: PDB
REMARK 900 RELATED ID: 3E1V   RELATED DB: PDB
DBREF  3E1W A    1   229  UNP    P45148   CAN_HAEIN        1    229
DBREF  3E1W B    1   229  UNP    P45148   CAN_HAEIN        1    229
SEQADV 3E1W ASN A   44  UNP  P45148    ASP    44 ENGINEERED
SEQADV 3E1W ASN B   44  UNP  P45148    ASP    44 ENGINEERED
SEQRES   1 A  229  MET ASP LYS ILE LYS GLN LEU PHE ALA ASN ASN TYR SER
SEQRES   2 A  229  TRP ALA GLN ARG MET LYS GLU GLU ASN SER THR TYR PHE
SEQRES   3 A  229  LYS GLU LEU ALA ASP HIS GLN THR PRO HIS TYR LEU TRP
SEQRES   4 A  229  ILE GLY CYS SER ASN SER ARG VAL PRO ALA GLU LYS LEU
SEQRES   5 A  229  THR ASN LEU GLU PRO GLY GLU LEU PHE VAL HIS ARG ASN
SEQRES   6 A  229  VAL ALA ASN GLN VAL ILE HIS THR ASP PHE ASN CYS LEU
SEQRES   7 A  229  SER VAL VAL GLN TYR ALA VAL ASP VAL LEU LYS ILE GLU
SEQRES   8 A  229  HIS ILE ILE ILE CYS GLY HIS THR ASN CYS GLY GLY ILE
SEQRES   9 A  229  HIS ALA ALA MET ALA ASP LYS ASP LEU GLY LEU ILE ASN
SEQRES  10 A  229  ASN TRP LEU LEU HIS ILE ARG ASP ILE TRP PHE LYS HIS
SEQRES  11 A  229  GLY HIS LEU LEU GLY LYS LEU SER PRO GLU LYS ARG ALA
SEQRES  12 A  229  ASP MET LEU THR LYS ILE ASN VAL ALA GLU GLN VAL TYR
SEQRES  13 A  229  ASN LEU GLY ARG THR SER ILE VAL LYS SER ALA TRP GLU
SEQRES  14 A  229  ARG GLY GLN LYS LEU SER LEU HIS GLY TRP VAL TYR ASP
SEQRES  15 A  229  VAL ASN ASP GLY PHE LEU VAL ASP GLN GLY VAL MET ALA
SEQRES  16 A  229  THR SER ARG GLU THR LEU GLU ILE SER TYR ARG ASN ALA
SEQRES  17 A  229  ILE ALA ARG LEU SER ILE LEU ASP GLU GLU ASN ILE LEU
SEQRES  18 A  229  LYS LYS ASP HIS LEU GLU ASN THR
SEQRES   1 B  229  MET ASP LYS ILE LYS GLN LEU PHE ALA ASN ASN TYR SER
SEQRES   2 B  229  TRP ALA GLN ARG MET LYS GLU GLU ASN SER THR TYR PHE
SEQRES   3 B  229  LYS GLU LEU ALA ASP HIS GLN THR PRO HIS TYR LEU TRP
SEQRES   4 B  229  ILE GLY CYS SER ASN SER ARG VAL PRO ALA GLU LYS LEU
SEQRES   5 B  229  THR ASN LEU GLU PRO GLY GLU LEU PHE VAL HIS ARG ASN
SEQRES   6 B  229  VAL ALA ASN GLN VAL ILE HIS THR ASP PHE ASN CYS LEU
SEQRES   7 B  229  SER VAL VAL GLN TYR ALA VAL ASP VAL LEU LYS ILE GLU
SEQRES   8 B  229  HIS ILE ILE ILE CYS GLY HIS THR ASN CYS GLY GLY ILE
SEQRES   9 B  229  HIS ALA ALA MET ALA ASP LYS ASP LEU GLY LEU ILE ASN
SEQRES  10 B  229  ASN TRP LEU LEU HIS ILE ARG ASP ILE TRP PHE LYS HIS
SEQRES  11 B  229  GLY HIS LEU LEU GLY LYS LEU SER PRO GLU LYS ARG ALA
SEQRES  12 B  229  ASP MET LEU THR LYS ILE ASN VAL ALA GLU GLN VAL TYR
SEQRES  13 B  229  ASN LEU GLY ARG THR SER ILE VAL LYS SER ALA TRP GLU
SEQRES  14 B  229  ARG GLY GLN LYS LEU SER LEU HIS GLY TRP VAL TYR ASP
SEQRES  15 B  229  VAL ASN ASP GLY PHE LEU VAL ASP GLN GLY VAL MET ALA
SEQRES  16 B  229  THR SER ARG GLU THR LEU GLU ILE SER TYR ARG ASN ALA
SEQRES  17 B  229  ILE ALA ARG LEU SER ILE LEU ASP GLU GLU ASN ILE LEU
SEQRES  18 B  229  LYS LYS ASP HIS LEU GLU ASN THR
HET     ZN  A 230       1
HET     ZN  B 230       1
HETNAM      ZN ZINC ION
FORMUL   3   ZN    2(ZN 2+)
FORMUL   5  HOH   *13(H2 O)
HELIX    1   1 PRO A   48  THR A   53  1                                   6
HELIX    2   2 VAL A   66  GLN A   69  5                                   4
HELIX    3   3 ASP A   74  VAL A   87  1                                  14
HELIX    4   4 CYS A  101  ALA A  109  1                                   9
HELIX    5   5 GLY A  114  HIS A  130  1                                  17
HELIX    6   6 HIS A  130  LEU A  137  1                                   8
HELIX    7   7 SER A  138  GLY A  159  1                                  22
HELIX    8   8 THR A  161  ARG A  170  1                                  10
HELIX    9   9 SER A  197  ILE A  214  1                                  18
HELIX   10  10 PRO B   48  THR B   53  1                                   6
HELIX   11  11 ASP B   74  VAL B   87  1                                  14
HELIX   12  12 CYS B  101  ALA B  109  1                                   9
HELIX   13  13 GLY B  114  HIS B  130  1                                  17
HELIX   14  14 HIS B  130  LEU B  137  1                                   8
HELIX   15  15 GLU B  140  ARG B  160  1                                  21
HELIX   16  16 THR B  161  ARG B  170  1                                  10
HELIX   17  17 SER B  197  SER B  213  1                                  17
SHEET    1   A 5 LEU A  60  ASN A  65  0
SHEET    2   A 5 TYR A  37  CYS A  42  1  N  TRP A  39   O  HIS A  63
SHEET    3   A 5 HIS A  92  HIS A  98  1  O  ILE A  94   N  LEU A  38
SHEET    4   A 5 SER A 175  ASP A 182  1  O  HIS A 177   N  ILE A  95
SHEET    5   A 5 GLY A 186  PHE A 187 -1  O  GLY A 186   N  ASP A 182
SHEET    1   B 5 LEU A  60  ASN A  65  0
SHEET    2   B 5 TYR A  37  CYS A  42  1  N  TRP A  39   O  HIS A  63
SHEET    3   B 5 HIS A  92  HIS A  98  1  O  ILE A  94   N  LEU A  38
SHEET    4   B 5 SER A 175  ASP A 182  1  O  HIS A 177   N  ILE A  95
SHEET    5   B 5 MET A 194  ALA A 195 -1  O  ALA A 195   N  LEU A 176
SHEET    1   C 5 LEU B  60  ASN B  65  0
SHEET    2   C 5 TYR B  37  CYS B  42  1  N  TRP B  39   O  PHE B  61
SHEET    3   C 5 HIS B  92  HIS B  98  1  O  ILE B  94   N  ILE B  40
SHEET    4   C 5 SER B 175  TYR B 181  1  O  HIS B 177   N  ILE B  95
SHEET    5   C 5 MET B 194  ALA B 195 -1  O  ALA B 195   N  LEU B 176
LINK         SG  CYS A  42                ZN    ZN A 230     1555   1555  2.29
LINK         NE2 HIS A  98                ZN    ZN A 230     1555   1555  2.04
LINK         SG  CYS A 101                ZN    ZN A 230     1555   1555  2.27
LINK         SG  CYS B  42                ZN    ZN B 230     1555   1555  2.31
LINK         NE2 HIS B  98                ZN    ZN B 230     1555   1555  2.06
LINK         SG  CYS B 101                ZN    ZN B 230     1555   1555  2.30
SITE     1 AC1  5 CYS A  42  ASN A  44  HIS A  98  CYS A 101
SITE     2 AC1  5 HOH A 231
SITE     1 AC2  5 CYS B  42  ASN B  44  HIS B  98  CYS B 101
SITE     2 AC2  5 HOH B 232
CRYST1   78.450   78.450  182.104  90.00  90.00  90.00 P 41 21 2    16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.012747  0.000000  0.000000        0.00000
SCALE2      0.000000  0.012747  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005491        0.00000
      
PROCHECK
Go to PROCHECK summary
 References