UniProt functional annotation for Q9RT63



	UniProt functional annotation for Q9RT63

UniProt code: Q9RT63.

Organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).

Taxonomy: Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus.

Function: DNA-dependent ATPase (ssDNA better than dsDNA) and ATP- dependent 5'-3' DNA helicase. Appears to move along DNA in single base steps, powered by hydrolysis of 1 molecule of ATP. Has low processivity; short (20 bp) substrates with 5'-overhangs or forked ends are the best substrates, is much less efficient on 52 or 76 bp substrates with 5'- overhangs. The presence of single-stranded DNA- binding protein (SSB) increases unwinding 4-5 fold. Has no activity on blunt DNA or DNA with 3'-overhangs. Requires at least 10 bases of 5'- ssDNA for helicase activity. {ECO:0000269|PubMed:15466873, ECO:0000269|PubMed:19490894}.

Catalytic activity: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000255|HAMAP-Rule:MF_01488};

Biophysicochemical properties: pH dependence: Optimum pH is 6.3 to 6.5 for unwinding of a 56 bp substrate and DNA hairpin binding. {ECO:0000269|PubMed:15466873};

Miscellaneous: There are no RecB or RecC orthologs in this organism.

Similarity: Belongs to the RecD family. RecD-like subfamily. {ECO:0000255|HAMAP-Rule:MF_01488}.

Annotations taken from UniProtKB at the EBI.


Organism:		Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
Taxonomy:		Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus.



Function:		DNA-dependent ATPase (ssDNA better than dsDNA) and ATP- dependent 5'-3' DNA helicase. Appears to move along DNA in single base steps, powered by hydrolysis of 1 molecule of ATP. Has low processivity; short (20 bp) substrates with 5'-overhangs or forked ends are the best substrates, is much less efficient on 52 or 76 bp substrates with 5'- overhangs. The presence of single-stranded DNA- binding protein (SSB) increases unwinding 4-5 fold. Has no activity on blunt DNA or DNA with 3'-overhangs. Requires at least 10 bases of 5'- ssDNA for helicase activity. {ECO:0000269\|PubMed:15466873, ECO:0000269\|PubMed:19490894}.


Catalytic activity:		Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000255\|HAMAP-Rule:MF_01488};
Biophysicochemical properties:		pH dependence: Optimum pH is 6.3 to 6.5 for unwinding of a 56 bp substrate and DNA hairpin binding. {ECO:0000269\|PubMed:15466873};
Miscellaneous:		There are no RecB or RecC orthologs in this organism.
Similarity:		Belongs to the RecD family. RecD-like subfamily. {ECO:0000255\|HAMAP-Rule:MF_01488}.