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PDBsum entry 3e1s
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* Residue conservation analysis
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Embo J
27:2222-2229
(2008)
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PubMed id:
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DNA binding to RecD: role of the 1B domain in SF1B helicase activity.
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K.Saikrishnan,
S.P.Griffiths,
N.Cook,
R.Court,
D.B.Wigley.
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ABSTRACT
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The molecular mechanism of superfamily 1Balpha helicases remains unclear. We
present here the crystal structure of the RecD2 helicase from Deinococcus
radiodurans at 2.2-A resolution. The structure reveals the folds of the 1B and
2B domains of RecD that were poorly ordered in the structure of the Escherichia
coli RecBCD enzyme complex reported previously. The 2B domain adopts an SH3 fold
which, although common in eukaryotes, is extremely rare in bacterial systems. In
addition, the D. radiodurans RecD2 structure has aided us in deciphering lower
resolution (3.6 A) electron density maps for the E. coli RecBCD enzyme in
complex with a long DNA substrate that interacts with the RecD subunit. Taken
together, these structures indicated an important role for the 1B domain of
RecD, a beta-hairpin that extends from the surface of the 1A domain and
interacts with the DNA substrate. On the basis of these structural data, we
designed a mutant RecD2 helicase that lacks this pin. The 'pin-less' mutant
protein is a fully active ssDNA-dependent ATPase but totally lacks helicase
activity.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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D.B.Wigley
(2012).
Bacterial DNA repair: recent insights into the mechanism of RecBCD, AddAB and AdnAB.
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Nat Rev Microbiol,
11,
9.
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C.G.Wu,
C.Bradford,
and
T.M.Lohman
(2010).
Escherichia coli RecBC helicase has two translocase activities controlled by a single ATPase motor.
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Nat Struct Mol Biol,
17,
1210-1217.
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L.Dostál,
and
J.F.Schildbach
(2010).
Single-stranded DNA binding by F TraI relaxase and helicase domains is coordinately regulated.
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J Bacteriol,
192,
3620-3628.
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M.C.Unciuleac,
and
S.Shuman
(2010).
Characterization of the mycobacterial AdnAB DNA motor provides insights into the evolution of bacterial motor-nuclease machines.
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J Biol Chem,
285,
2632-2641.
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S.Lang,
K.Gruber,
S.Mihajlovic,
R.Arnold,
C.J.Gruber,
S.Steinlechner,
M.A.Jehl,
T.Rattei,
K.U.Fröhlich,
and
E.L.Zechner
(2010).
Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases.
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Mol Microbiol,
78,
1539-1555.
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L.P.Blair,
A.J.Tackett,
and
K.D.Raney
(2009).
Development and evaluation of a structural model for SF1B helicase Dda.
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Biochemistry,
48,
2321-2329.
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M.L.Bochman,
and
A.Schwacha
(2009).
The Mcm complex: unwinding the mechanism of a replicative helicase.
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Microbiol Mol Biol Rev,
73,
652-683.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
