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PDBsum entry 3dyb
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References listed in PDB file
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Key reference
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Title
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High-Resolution structure of proteinase k cocrystallized with digalacturonic acid.
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Authors
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S.B.Larson,
J.S.Day,
C.Nguyen,
R.Cudney,
A.Mcpherson.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009,
65,
192-198.
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PubMed id
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Abstract
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Proteinase K, a subtilisin-like fungal protease, was crystallized from a
cocktail of small molecules containing digalacturonic acid (DGA). The crystal
structure was determined to 1.32 A resolution and refined to an R factor of
0.158. The final model contained, beside the protein, two calcium ions, 379
water molecules, a molecule of DGA and a partially occupied HEPES molecule. The
DGA molecule has one sugar moiety disposed exactly on a crystallographic twofold
axis; the second ring was not observed. The DGA molecule is bound to two protein
molecules across the twofold axis through hydrogen-bonding networks involving
Ser150 and water molecules. One of the calcium-ion sites has not been reported
previously. This study further illustrates the involvement of small molecules in
the crystallization of macromolecules through their ability to form
intermolecular lattice interactions.
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Secondary reference #1
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Title
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Progress in the development of an alternative approach to macromolecular crystallization
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Authors
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S.B.Larson,
J.S.Day,
C.Nguyen,
R.Cudney,
A.Mcpherson.
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Ref.
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CRYST GROWTH DES, 2008,
8,
3038-3052.
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